植物学报2025，Vol.60Issue(4)：597-610,14.DOI:10.11983/CBB24151
菊花品种万代风光再生及遗传转化体系的建立
Establishment of Regeneration and Genetic Transformation System for Chrysanthemum × morifolium'Wandai Fengguang'
摘要
Abstract
INTRODUCTION:Chrysanthemum × morifolium is one of the ten most famous traditional flowers in China,and it has a rich variety of cultivars with diverse floral colour and shapes.However,varieties with blue floral colour have not been found in the natural chrysanthemum,therefore,breeding blue chrysanthemums has always been a goal pursued by re-searchers. RATIONALE:The total flavonoid extract of C.× morifolium'Wandai Fengguang'could turn blue when adding appropriate concentration of Fe3+,and its living petal cells could also turn blue with the participation of Fe3+,which proved the feasi-bility of breeding blue chrysanthemums with Fe3+.Meanwhile,C.× morifolium'Wandai Fengguang'can bloom both in summer and autumn,with early flowering and long flowering period,which is also an important material for the study of flowering period.Therefore,in order to cultivate blue chrysanthemums and achieve the targeted improvement of flowering period,it is particularly important to establish an efficient and stable regeneration and genetic transformation system for the C.× morifolium'Wandai Fengguang'.However,chrysanthemum has a long history of cultivation and complex genetic background,so the regeneration and genetic transformation system is not universal among different varieties. RESULTS:In this study,C.× morifolium'Wandai Fengguang'was used as the experimental material to study the effects of different explant types with different combinations of plant growth regulators on its regeneration,and to investigate the effects of relevant factors on the efficiency of genetic transformation with the Agrobacterium-mediated genetic transfor-mation method.The experimental results showed that the most suitable explants for the regeneration of C.× morifolium'Wandai Fengguang'was the transverse thin cell layers(tTCLs),and the optimal culture medium was MS+1.5 mg·L-16-BA+0.6 mg·L-1 NAA.The highest differentiation rate was 70.06％and an adventitious bud coefficient was 3.37.The kanamycin selection pressures for the differentiation of the tTCLs and the adventitious bud rooting were 7.5 mg·L-1 and 5.0 mg·L-1,respectively.The optimal procedure for genetic transformation was pre-culture for 1 day,OD600=0.8,treatment for 5 minutes,and co-culture in the dark for 3 days.Fifteen resistant plantlets were screened on kanamycin medium,and two positive plantlets were confirmed by PCR amplification,with a transformation efficiency of 13.33％. CONCLUSION:This study laid the foundation for the gene function analysis and targeted improvement molecular breeding of chrysanthemum by using this kind of unique variety resource,and provided reference for the establishment of regeneration and transformation system for other chrysanthemum varieties.关键词
菊花/外植体/遗传转化体系/再生体系/茎间薄层Key words
chrysanthemum/explant/genetic transformation system/regeneration system/transverse thin cell layers引用本文复制引用
李晶晶,李艳飞,王安琪,王佳颖,邓成燕,卢敏,马剑英,戴思兰..菊花品种万代风光再生及遗传转化体系的建立[J].植物学报,2025,60(4):597-610,14.基金项目
国家自然科学基金(No.32171849,No.32371948)和福建省科技计划(No.2022S0004) （No.32171849,No.32371948）
