植物学报2025，Vol.60Issue(4)：611-620,10.DOI:10.11983/CBB24113
西印度醋栗组培快繁技术初探
A Preliminary Study on Tissue Culture and Rapid Propagation Technology of Phyllanthus acidus
摘要
Abstract
INTRODUCTION:A preliminary tissue culture system for Phyllanthus acidus was established. RATIONALE:In this study,the stem tips of P.acidus were used as explants,and the schemes of primary culture,se-condary proliferation culture and rooting culture were screened. RESULTS:The results showed that the optimal medium for primary culture was MS+2.0 mg·L-1 6-BA+0.2 mg·L-1 NAA,and the induced germination rate of explants reached 81.11％.The optimal medium for subculture was MS+1.0 mg·L-16-BA+0.2 mg·L-1 IBA,and the proliferation coefficient was 1.86.The optimal medium for rooting was MS+1.5 mg·L-1 IBA,and the rooting rate reached 83.00％.After 7 days of cultivation,the plantlets were transplanted with perlite,peat soil and humus=1∶1∶1(v/v/v),and the survival rate was 90％. CONCLUSION:The most suitable medium for primary culture using the stem tip of P.acidus as explant was MS me-dium and the phytohormones were 2.0 mg·L-1 6-BA and 0.2 mg·L-1 NAA;the optimal phytohormones for subculture were 1.0 mg·L-1 6-BA and 0.2 mg·L-1 IBA;the optimal phytohormone for rooting culture was 1.5 mg·L-1 IBA.关键词
西印度醋栗/组织培养/快速繁殖Key words
Phyllanthus acidus/tissue culture/rapid propagation引用本文复制引用
李彤,李楚然,张芷瑜,付晓熳,刘云,张颖君,杨力颖,赵平..西印度醋栗组培快繁技术初探[J].植物学报,2025,60(4):611-620,10.基金项目
国家自然科学基金(No.32270426,No.82074124) （No.32270426,No.82074124）
