植物研究2025，Vol.45Issue(4)：491-503,13.DOI:10.7525/j.issn.1673-5102.2025.04.003
钩藤水杨酸响应基因UrSTR4参与钩藤碱合成
The Salicylic Acid Responsive UrSTR4 Gene is Involved in Rhynchophylline Biosynthesis in Uncaria rhynchophylla
摘要
Abstract
Uncaria rhynchophylla is a traditional Chinese medicinal herb,whose active components rhynchophylline(RIN)and isorhynchophylline(IRN)exhibit remarkable pharmacological activities.Strictosidine synthase(STR)is the key enzyme for RIN and IRN biosynthesis,yet the functional divergence of STR gene family in U.rhynchophylla remains unclear.Utilizing transcriptome data of U.rhynchophylla seedlings under shading and ethylene treatments,27 STR genes(UrSTR1-UrSTR27)were systematically identified in U.rhynchophylla.Evolutionary analysis revealed significant expansion of UrSTR gene family,suggesting functional diversification in response to environmental cues.Exogenous salicylic acid(SA)treatment(0.6 mmol·L-1)markedly increased RIN and IRN contents,while upregulating UrSTR4 expression level by 30.6-fold.Multiple sequence alignment showed UrSTR4 lacked the conserved Glu-309 residue but shared hydroxyl-containing residues with Camptotheca STR3.An efficient transient transformation system(8 kPa pressure,1-min Agrobacterium infection)was established by using plant living transformation instrument.The transient overexpression of UrSTR4 enhanced RIN and IRN contents by 1.96-and 1.82-fold,respectively.This study identified new targets for metabolic regulation of bioactive compounds in U.rhynchophylla and the established transient transformation system provided technical support for functional research on UrSTR genes.关键词
钩藤/异胡豆苷合成酶/水杨酸/瞬时转化/单萜吲哚生物碱Key words
Uncaria rhynchophylla/strictosidine synthase/salicylic acid/transient transformation/monoter-penoid indole alkaloids分类
农业科技引用本文复制引用
许国菊,郭飘雪,朱寻然,杨兹梅,成环宇,强玮..钩藤水杨酸响应基因UrSTR4参与钩藤碱合成[J].植物研究,2025,45(4):491-503,13.基金项目
贵州省基础研究(自然科学)项目(黔科合基础-ZK[2022]一般096,黔科合基础MS[2025]667,黔科合基础-ZK[2024]一般502) （自然科学）
国家自然科学基金项目(32460146). （32460146）
