植物研究2025，Vol.45Issue(4)：546-557,12.DOI:10.7525/j.issn.1673-5102.2025.04.007
香果树种子萌发的光照需求及其转录组响应分析
Light Requirements for Germination of Emmenopterys henryi Oliv.Seeds and Transcriptome Response Analysis
摘要
Abstract
This study aimed to investigate the light duration required for seed germination of Emmenopterys henryi Oliv.,an endangered species endemic to China,and to explore the correlation between morphological traits and germination rate of seeds under different light durations.Using E.henryi seeds as material,this study compared the differences in germination rates under light treatments of 0,1,2(8 h/1st day+4 h/2nd day),2,4,6,8 d.The correlation between seed morphology and germination time was also analyzed.In addition,transcriptome sequencing and analysis using high-throughput technology were perfomed on E.henryi seeds subjected to dark treatment(2 d)and basic light exposure(the shortest light duration required for a germination rate of≥50%,2 d).Results showed that,under light treatment conditions,the germination rates increased with extension of light duration.For the seeds subjected to the 8 d light treatment,the germination rate reached 98.89%after 10 d of cultivation.The analysis of correlation between seed morphological characteristics and germination time showed that the length of seed kernel and length-to-width ratio of seed kernel were significantly negatively correlated with germination time(P<0.05).Transcriptomic responses of the seeds following dark treatment(2 d)and basic light treatments(2 d)indicated 4 657 differentially expressed genes(DEGs),including 2 950 upregulated and 1 707 downregulated genes.Among the 185 transcription factors identified,all members of the HSF family were downregulated in the light treatment group.GO terms related to protein metabolism were significantly enriched.DEGs were significantly enriched in KEGG pathways(P-adjust<0.05)related to embryo development and seedling stress resistance,including diterpenoid biosynthesis(ko00904),cysteine and methionine metabolism(ko00270),and plant-pathogen interaction(ko04626).In summary,E.henryi seeds with larger kernels germinated earlier,and light supplied for two days was sufficient to meet the basic germination requirements of the seeds.This study provided a theoretical basis for elucidating the mechanisms underlying the abnormal population structure of wild E.henryi populations and for promoting its population restoration in natural conditions.关键词
香果树/种子/光照/萌发/转录组Key words
Emmenopterys henryi/seed/light/germination/transcriptome分类
生物科学引用本文复制引用
程薪宇,郭梦桥,官海云,茹剑,白琰,郭连金..香果树种子萌发的光照需求及其转录组响应分析[J].植物研究,2025,45(4):546-557,12.基金项目
国家自然科学基金地区科学基金项目(32060336,31860200,31360145) （32060336,31860200,31360145）
江西省教育厅科学技术研究项目(GJJ211719,GJJ211723). （GJJ211719,GJJ211723）
