植物研究2025，Vol.45Issue(4)：558-568,11.DOI:10.7525/j.issn.1673-5102.2025.04.008
濒危植物岩生鼠尾草的组培快繁技术
Tissue Culture and Rapid Propagation of Endangered Salvia petrophila
摘要
Abstract
To establish the rapid propagation system of tissue culture of an endangered plant Salvia petrophila,the disinfectant concentration and duration in the sterilization process,as well as the regulation of hormone types and concentrations on seed germination,the subculture and preliferation of stem segments with axillary buds at the base,induction and differentiation of callus from stem segments,rooting,hardening off and transplanting were investigated respectively.The results showed that(1)the appropriate sterilization method for S.petrophila seeds was 75%alcohol treatment for 30 s,followed by 5%sodium hypochlorite solution for 8 min,achieving a germination rate of 40.83%.(2)The appropriate medium for seed germination was MS supplemented with 1.0 mg·L-1 6-BA and 0.1 mg·L-1 NAA,resulting in a germination rate of 71.12%.(3)For subculture proliferation,the appropriate medium was MS with 1.0 mg·L-1 6-BA and 0.1 mg·L-1 NAA,yielding a proliferation coefficient of 5.5.(4)For stem segments,the appropriate callus induction medium was MS+1.0 mg·L-1 6-BA+1.0 mg·L-1 2,4-D,with an induction rate of 97.10%.The appropriate differentiation medium for callus induced from stem segments was MS+1.5 mg·L-1 6-BA+0.1 mg·L-1 NAA.(5)The appropriate basic medium for adventitious bud rooting was 1/2MS with 1.0 mg·L-1 NAA,achieving a rooting coefficient of 99.11%.(6)After hardening off,the rooted seedlings of S.petrophila were transplanted into a mixed substrate of peat,perlite,and vermiculite in a volume ratio of 1∶1∶1,resulting in a survival rate of 85.5%.The results might lay a technical foundation for the species conservation and resource utilization of S.petrophila,and also provided valuable ideas for the rapid propagation techniques of other Salvia species.关键词
岩生鼠尾草/濒危植物/组织培养/快速繁殖Key words
Salvia petrophila/endangered plant/tissue culture/rapid propagation分类
农业科技引用本文复制引用
王莹莹,余登利,邱风进,晏融融,胡国雄..濒危植物岩生鼠尾草的组培快繁技术[J].植物研究,2025,45(4):558-568,11.基金项目
国家自然科学基金地区科学基金项目(32060048) （32060048）
贵州省科技计划项目(黔科合中引地[2023]029). （黔科合中引地[2023]029）
