中国现代中药2025，Vol.27Issue(7)：1197-1206,10.DOI:10.13313/j.issn.1673-4890.20241212008
中医方药整体作用靶标研究模式探讨
Research Modes of the Functional Targets of Traditional Chinese Medicine Prescriptions
摘要
Abstract
Adhering to the core characteristics of traditional Chinese medicine(TCM)prescriptions,including their integrity and structure,is a key prerequisite for revealing their therapeutic mechanisms and multi-target synergistic effects.TCM prescriptions exert medicinal effects by interacting with the target proteins.However,the current research model mainly focuses on individual components,which cannot reflect prescription integrity and is thus insufficient to explain the therapeutic effects and mechanisms of TCM prescriptions.In recent years,the development of unmodified ligand detection technology based on high-throughput mass spectrometry has provided strong support for the identification and affinity detection of the overall action targets of TCM prescriptions.Under the guidance of the holistic view of TCM,this paper proposes a research model for the overall action targets of TCM prescriptions and their components.This model comprises five modules:target identification based on TCM prescriptions,target-effector association,revealing TCM efficacy through effectors,efficacy verification in clinical practice,and inheritance and innovation.The unmodified ligand detection technology basing on mass spectrometry is used for the discovery,identification,and affinity detection of target proteins in a high-throughput way.Then,bioinformatics is employed to analyze the biological big data generated under TCM prescription treatment and select the functional molecules related to the action of TCM prescriptions.According to the theory of signal transduction,targets are associated with functional molecules,and the functional molecules of TCM prescriptions in regulating diseases are precisely analyzed.This could help comprehensively construct the functional network of TCM prescriptions in treating diseases and explore their potential new effects.Further,clinical practice is employed to verify the innovative efficacy of TCM prescriptions that is obtained from the experiments,and thus the precise clinical positioning is achieved.In summary,the in-depth research on the innovative efficacy,scientific concepts,precise clinical position,and therapeutic mechanisms of TCM prescriptions could promote the inheritance and innovation of the theory of TCM prescriptions.关键词
中医方药/中药靶标/整体作用/高通量质谱/配体免修饰技术Key words
traditional Chinese medicine(TCM)prescriptions/targets of TCM/holistic effect/high-throughput mass spectrometry/unmodified ligand detection technology分类
医药卫生引用本文复制引用
邓欣祺,奇蓉莹,王春国..中医方药整体作用靶标研究模式探讨[J].中国现代中药,2025,27(7):1197-1206,10.基金项目
国家自然科学基金项目(82404932,82374076,81703744) （82404932,82374076,81703744）
中国中医科学院中药研究所人才引进项目(ZXKT22059,ZZ16-XRZ-071,ZZ17-YQ-023) （ZXKT22059,ZZ16-XRZ-071,ZZ17-YQ-023）
北京市自然科学基金青年科学基金项目(7244493) （7244493）
北京中医药大学实验技术(操作规程)标准化研究项目(2023-syjs-01) （操作规程）
