中国现代中药2025，Vol.27Issue(7)：1207-1216,中插1-中插4,14.DOI:10.13313/j.issn.1673-4890.20240715006
基于热蛋白质组学技术的三七水煎液治疗脑病作用靶标及潜在新功效研究
Thermal Proteome Profiling Reveals the Targets and Potential New Efficacy of Notoginseng Radix et Rhizoma Decoction in Treating Encephalopathy
摘要
Abstract
Objective:Based on the research model for integrative effect of TCM,which consist of modules including"detecting targets of the drug","targeted-regulated molecule association","obtaining the efficacy with the regulated molecule","clinical validation of efficacy",and"inheritance and innovation of TCM",this study gave precise explanation for the function of the Notoginseng Radix et Rhizoma decoction in brain diseases treatment,,and reveals its potential new efficacy..Methods:Thermal proteome profiling was employed for high-throughput screening of the overall targets of this decoction in the mouse brain tissue.The Ingenuity Pathway Analysis(IPA)database was used for the association analysis of the targets of Notoginseng Radix et Rhizoma decoction and the downstream target proteins.Genvestigator was further used to mine and analyze the target-associated diseases.Results:In this study,15 direct targets and 17 indirect targets of Notoginseng Radix et Rhizoma decoction were screened out.The analyses via IPA and Genvestigator showed that some of these targets were involved in the treatment of ischemic stroke and Alzheimer's disease and they were capable of repairing the nerve damage through the new efficacy of neural regeneration.Conclusion:In this study,we elucidate the mechanism of Notoginseng Radix et Rhizoma decoction in treating encephalopathies from the perspective of target and revealed the potential efficacy of this decoction in repairing nerve damage through nerve regeneration.关键词
中药靶标/三七/水煎液/热蛋白质组学/作用机制/脑病/神经再生Key words
Chinese medicine target/Notoginseng Radix et Rhizoma/decoction/thermal proteome profiling/mechanism of action/encephalopathy/nerve regeneration分类
医药卫生引用本文复制引用
陈恒,董婧,邓欣祺,王春国,饶沁玲,黄智兴,张鸽,何蓉格,刘嘉彤,梁馨宁,侯毓扬,孔乔慧..基于热蛋白质组学技术的三七水煎液治疗脑病作用靶标及潜在新功效研究[J].中国现代中药,2025,27(7):1207-1216,中插1-中插4,14.基金项目
国家自然科学基金项目(82374076,81703744) （82374076,81703744）
北京市科学技术协会2023-2025年度青年人才托举工程项目(BYESS2023354) （BYESS2023354）
北京市自然科学基金青年科学基金项目(7244493) （7244493）
北京中医药大学实验技术(操作规程)标准化研究项目(2023-syjs-01) （操作规程）
