中国现代中药2025，Vol.27Issue(7)：1217-1227,中插5-中插6,13.DOI:10.13313/j.issn.1673-4890.20241111003
基于热蛋白质组学技术的芩栀猪胆方治疗脑病作用靶标及潜在新功效研究
Exploration of Targets and Potential New Efficacies of Qinzhi Zhudan Decoction in Encephalopathy Based on Thermal Proteome Profiling
摘要
Abstract
Objective:Based on the research model for integrative effect of TCM,which consist of modules including"detecting targets of the drug","targeted-regulated molecule association","obtaining the efficacy with the regulated molecule","clinical validation of efficacy",and"inheritance and innovation of TCM",this study gave precise explanation for the function of Qinzhi Zhudan Decoction in brain diseases treatment,and reveals its potential new efficacy.Methods:Thermal proteome profiling(TPP)was employed to perform high-throughput screening of the overall targets of Qinzhi Zhudan Decoction in mouse brain tissue.The ingenuity pathway analysis(IPA)database was used to analyze the associations between the targets of Qinzhi Zhudan Decoction and downstream target proteins.Furthermore,the Genevestigator platform was utilized to explore diseases associated with these targets.Results:A total of 20 direct targets and 18 indirect targets of Qinzhi Zhudan Decoction were identified.Joint analysis using the IPA database and Genevestigator platform revealed that these targets were involved in the treatment of Alzheimer's disease,ischemic stroke,depression,vascular dementia,and other conditions.Additionally,the analysis suggested that Qinzhi Zhudan Decoction may have potential new efficacies in the treatment of ataxia and brain glioma.Conclusion:This study elucidates the mechanism of Qinzhi Zhudan Decoction in the treatment of brain disorders from the perspective of target engagement,further supports its potential therapeutic value in central nervous system diseases,including ataxia and glioma.关键词
中药靶标/芩栀猪胆方/热蛋白质组学/名医名方/脑病Key words
traditional Chinese medicine target/Qinzhi Zhudan Decoction/thermal proteome profiling/famous TCM prescription/encephalopathy分类
医药卫生引用本文复制引用
黄智兴,奇蓉莹,李沐萱,姜淑玮,孟熙婧,王庆国,邓欣祺,马涛,王春国,陈恒,饶沁玲,张鸽,何蓉格,胡子怡,吕嘉瑞..基于热蛋白质组学技术的芩栀猪胆方治疗脑病作用靶标及潜在新功效研究[J].中国现代中药,2025,27(7):1217-1227,中插5-中插6,13.基金项目
国家自然科学基金项目(82404932,82374076,81703744) （82404932,82374076,81703744）
北京市科学技术协会2023-2025年度青年人才托举工程项目(BYESS2023354) （BYESS2023354）
北京市自然科学基金青年科学基金项目(7244493) （7244493）
北京中医药大学实验技术(操作规程)标准化研究项目(2023-syjs-01) （操作规程）
国家重点研发计划中医药现代化专项(2023YFC3504100) （2023YFC3504100）
