中国现代中药2025，Vol.27Issue(7)：1228-1237,中插7,11.DOI:10.13313/j.issn.1673-4890.20241112003
基于热蛋白质组学技术的疏风解毒胶囊作用靶标及抗病毒机制研究
Thermal Proteome Profiling Reveals the Targets and Antiviral Mechanism of Shufeng Jiedu Capsules
摘要
Abstract
Objective:Based on the research model for integrative effect of TCM,which consist of modules including"detecting targets of the drug","targeted-regulated molecule association","obtaining the efficacy with the regulated molecule","clinical validation of efficacy",and"inheritance and innovation of TCM",this study gave investigation in the target and new mechanism of the Shufeng Jiedu Capsules(SFJDC)in anti-H1N1 virus infection.Methods:Animal experiments were conducted to assess the effects of SFJDC on H1N1 invasion in the lungs of mice.Thermal proteome profiling was performed for the high-throughput screening of the overall targets of SFJDC in BEAS-2B cells.Additionally,the Ingenuity Pathway Analysis(IPA)database was used for correlation analysis between the targets of SFJDC and disease-specific differentially expressed proteins.Results:SFJDC reduced the levels of viral RNA,lung index,and the expression of inflammatory factors such as interleukin(IL)-1,IL-6,and tumor necrosis factor-alpha in the lung tissue of virus-infected mice,demonstrating significant antiviral efficacy.We identified 150 direct targets and 66 indirect targets of SFJDC,of which 42 direct targets and 19 indirect targets were associated with antiviral efficacy and contributed therapeutically to various aspects of viral replication and infection.Furthermore,SFJDC may be involved in regulating several signaling pathways,including the interferon,mitogen-activated protein kinase/nuclear transcription factor-κB,Janus kinase 1-signal transducer and activator of transcription 1,Toll-like receptor,and endocytosis pathways,thereby exerting its antiviral effects.Conclusion:The"high-throughput target screening-target association-clinical application"research methodology of traditional Chinese medicine clarifies the mechanism underpinning the antiviral efficacy of SFJDC from the perspective of target.关键词
中药靶标/疏风解毒胶囊/热蛋白质组学/抗病毒/作用机制Key words
Chinese medicine target/Shufeng Jiedu Capsules/thermal proteome profiling/antiviral/mechanism of action分类
医药卫生引用本文复制引用
陈恒,申冰清,孙潇寒,李沐萱,陈远,邓欣祺,崔晓兰,王春国,胡子怡,饶沁玲,黄智兴,张鸽,何蓉格,郑浩宇,刘舒阳..基于热蛋白质组学技术的疏风解毒胶囊作用靶标及抗病毒机制研究[J].中国现代中药,2025,27(7):1228-1237,中插7,11.基金项目
国家自然科学基金项目(82141206) （82141206）
北京市自然科学基金项目(7244493) （7244493）
中国中医科学院中药研究所人才引进项目(ZXKT22059,ZZ16-XRZ-071,ZZ17-YQ-023) （ZXKT22059,ZZ16-XRZ-071,ZZ17-YQ-023）
北京中医药大学实验技术(操作规程)标准化研究项目(2023-syjs-01) （操作规程）
