中国现代中药2025，Vol.27Issue(7)：1245-1254,10.DOI:10.13313/j.issn.1673-4890.20241224005
肾茶的化学成分及其抑制α-葡萄糖苷酶活性研究
Chemical Constituents of Clerodendranthus spicatus and Inhibitory Activity against α-Glucosidase
摘要
Abstract
Objective:The chemical constituents of Clerodendranthus spicatus(Thunb.)C.Y.Wu ex H.W.Li were isolated,and their inhibitory activities against α-glucosidase were studied.Methods:The compounds were isolated and purified by silica gel chromatography,Sephadex LH-20 gel chromatography,ODS reversed-phase chromatography,and semi-preparative liquid chromatography,and their structures were identified on the basis of the spectral data.At the same time,the inhibitory activity of the compounds against α-glucosidase was tested by 4-nitrophenyl-β-D-glucopyranoside(PNPG)method.Results:Twenty-four compounds were isolated and identified,namely asracsyn-2,3-dihydroxy-3-(3,4-dihydroxyphenyl)-propionicacid(1),luteolin(2),3-(3,4-dihydroxyphenyl)-acrylicacid-1-(3,4-dihydroxyphenyl)-2-methoxycarbonylethylester(3),methylrosmarinate(4),rosmarinicacid(5),oresbiusin A(6),orthosiphoicacid A(7),kaempferol-3-O-(2″-O-β-D-glycopyranosyl)-β-D-glucopyranoside(8),carnosic acid(9),ursolicacid(10),oleanolicacid(11),2,6-dimethoxy-p-benzoquinone(12),2-O-deacetylorthosiphol J(13),neoorthosiphol B(14),orthosiphol N(15),staminol C(16),benzoic acid(17),5,3′-dihydroxy-7,4′-dimethoxyflavone(18),eupatilin(19),valeric acid(20),veratric acid(21),3-indolecarboxaldehyde(22),(6R,7E,9S)-9-hydroxy-4,7-megastigmadiene-3-one(23),and orthosiphonone A(24).Conclusion:Among them,compounds 1,8,12,22,and 23 were isolated from this plant for the first time.Compounds 2-4,21,and 22 had potent α-glucosidase inhibition,and their IC50 were(45.76±0.01),(51.09±0.01),(41.08±0.03),(66.96±0.72),and(116.80±0.33)μg·mL-1,respectively.关键词
肾茶/化学成分/提取分离/结构鉴定/α-葡萄糖苷酶Key words
Clerodendranthus spicatus(Thunb.)C.Y.Wu ex H.W.Li/chemical constituent/extraction separation/structural identification/α-glucosidase分类
医药卫生引用本文复制引用
陈鑫,顾永哲,舒积成,梁永红,谭婷,罗云..肾茶的化学成分及其抑制α-葡萄糖苷酶活性研究[J].中国现代中药,2025,27(7):1245-1254,10.基金项目
国家自然科学基金项目(81760711,82160824,82260751) （81760711,82160824,82260751）
江西省中医药中青年骨干人才培养计划项目(1242100903) （1242100903）
江西中医药大学重点领域科技创新团队计划项目(CXTD22006) （CXTD22006）
