中国现代中药2025，Vol.27Issue(7)：1255-1261,7.DOI:10.13313/j.issn.1673-4890.20240617001
基于柱前衍生化高效液相色谱法的半夏中葡甘聚糖含量分析
Content Determination of Glucomannan in Pinellia ternata Based on HPLC Method with Pre-column Derivatization
摘要
Abstract
Objective:To establish a high performance liquid chromatography(HPLC)method with a pre-column derivatization to determine glucomannan content in Pinellia ternata(Thunb)Breit.by using 1-phenyl-3-methyl-5-pyrazolinone(PMP).Method:ChomCore AQ C18(250 mm×4.6 mm,5 μm)column was used with mobile phase A of acetonitrile and mobile phase B of 0.01 mol·L-1 ammonium acetate solution,and gradient elution was performed.The column temperature was 30℃;the volume flow rate was 0.8 mL·min-1,and the detection wavelength was 250 nm.The optimization of ultrasonic extraction conditions was conducted.An optimized method was used to detect the monosaccharide composition of hydrolyzed P.ternata and detect the D-mannose and D-glucose content in 13 P.ternata samples from different producing areas and three different processed products.Result:The optimal conditions for ultrasonic extraction were determined as follows:30 mL ultrapure water at 50℃for 60 minutes.The results of methodological validation showed that D-mannose and D-glucose showed good linear relationships within their mass concentration ranges(r≥0.99).The method also demonstrated satisfactory repeatability,precision,stability,and recovery rates,indicating suitability for detecting D-mannose and D-glucose in P.ternata.The monosaccharides hydrolyzed from P.ternata were mannose and glucose,with a D-mannose to D-glucose molar ratio ranging from(1.47±0.06)～(1.86±0.07),demonstrating the existence of glucomannans in P.ternata.Glucomannans were detected in all 13 P.ternata samples from various producing areas and in three processed products,with significant differences in content.Conclusion:The established method has the advantages of simplicity,high repeatability,and accuracy.The results demonstrate the presence of glucomannans in P.ternata and suggest that their content is influenced by the producing area and processing method,providing technical support for the composition analysis and quality control of P.ternata.关键词
半夏/葡甘聚糖/柱前衍生化/高效液相色谱法/D-葡萄糖/D-甘露糖Key words
Pinellia ternata(Thunb)Briet./glucomannan/pre-column derivatization/HPLC/D-glucose/D-mannose分类
医药卫生引用本文复制引用
付皎,魏璐,李金鑫,徐荣,王明星,吕建梅,冯书敏,刘大会..基于柱前衍生化高效液相色谱法的半夏中葡甘聚糖含量分析[J].中国现代中药,2025,27(7):1255-1261,7.基金项目
国家重点研发计划项目(2023YFC3503804) （2023YFC3503804）
财政部和农业农村部:国家中药材产业技术体系资助项目 （）
中央本级重大增减支项目(2060302) （2060302）
