中国现代中药2025，Vol.27Issue(7)：1262-1272,11.DOI:10.13313/j.issn.1673-4890.20241227001
基于UPLC-Q-TOF-MS/MS与定量指纹图谱的陈皮(樟头红)质量分析
Quality Analysis of Citri Reticulatae Pericarpium Based on UPLC-Q-TOF-MS/MS and Quantitative Fingerprinting
摘要
Abstract
Objective:To investigate the quality of Citri Reticulatae Pericarpium produced from Citrus reticulata'Zhangshuensis'based on quantitative fingerprinting and ultra-performance liquid chromatography-quadrupole-time of flight-tandem mass spectrometry(UPLC-Q-TOF-MS/MS).Methods:The fingerprints of 18 batches of Citri Reticulatae Pericarpium('Zhangshuensis')samples were obtained,and UPLC-Q-TOF-MS/MS was employed to identify the common peaks.The similarity analysis,cluster analysis,and orthogonal partial least squares-discriminant analysis were conducted to evaluate the quality of'Zhangshuensis'.Results:The fingerprint similarity of the 18 batches of samples ranged from 0.988 to 0.999.A total of 24 common peaks were identified in the reference fingerprint of'Zhangshuensis',among which 21 components were identified,including 10 polymethoxyflavonoids,4 flavone O-glycosides,2 flavone C-glycosides,3 phenylpropanoids,and 2 limonoids.On this basis,the chemical pattern recognition screened out two differential quality markers:hesperetin and 5-demethylnobiletin,which can distinguish'Zhangshuensis'from other varieties.Conclusion:This study establishes a quantitative fingerprinting method and identifies the common peaks of C.reticulata'Zhangshuensis'for the first time.This established method is stable and reliable and can distinguish'Zhangshuensis'from other cultivated varieties.The results can provide data reference and a scientific basis for the quality control,quality evaluation,and identification of'Zhangshuensis'.关键词
陈皮(樟头红)/定量指纹图谱/超高效液相色谱-四极杆飞行时间质谱法/化学模式识别/含量测定Key words
Citrus reticulata'Zhangshuensis'/quantitative fingerprinting/ultra-performance liquid chromatography-quadrupole-time of flight-tandem mass spectrometry(UPLC-Q-TOF-MS/MS)/chemical pattern recognition/content determination分类
医药卫生引用本文复制引用
甘佳攀,金红利,梁鑫淼,艾嘉浩,徐鑫,薛倩倩,邹佳芬,张旗,何思思,杨迎,付文艳..基于UPLC-Q-TOF-MS/MS与定量指纹图谱的陈皮(樟头红)质量分析[J].中国现代中药,2025,27(7):1262-1272,11.基金项目
江西省重大科技研发专项"揭榜挂帅"企业需求类项目(20223AAG02021) （20223AAG02021）
赣江新区科技计划项目(2022001) （2022001）
江西省重点研发计划项目(20224BBG72001) （20224BBG72001）
