中国现代中药2025，Vol.27Issue(7)：1283-1290,8.DOI:10.13313/j.issn.1673-4890.20250113006
降解铁皮石斛多糖的内生真菌筛选及产物组分分析
Screening of Endophytic Fungi Degrading Polysaccharides from Dendrobium officinale and Analysis of Product Components
摘要
Abstract
Objective:To screen potential strains of degradable polysaccharides from endophytic fungi using Dendrobium officinale polysaccharides(DOP)as the sole nutrient.Methods:Endophytic fungi capable of degrading crude DOP were screened using microbial liquid culture fermentation technology.The relative molecular weight,total sugar content,total protein content,uronic acid content,and monosaccharide composition of the degradation products were determined using high-performance liquid chromatography,phenol-sulfuric acid method,BCA method,m-hydroxybiphenyl method,and PMP pre-column derivatization method.The ultraviolet spectrum,infrared spectrum,and apparent structure of the degradation products were compared and analyzed.The antioxidant capacity of the degradation products was evaluated by 1,1-diphenyl-2-trinitrophenylhydrazine(DPPH)free radical scavenging assay.Results:Four endophytic fungi were found to degrade crude DOP-60%.Among them,the degradation product of strain D-25 had the smallest relative molecular mass(2.55×103)and the highest yield(76.7%).The monosaccharide composition of DOP-60%and the degradation products CDOP-3 to CDOP-6 were the same,with an increase in mannose content.Antioxidant activity studies showed that the DPPH free radical scavenging ability of CDOP-5 in the degradation products was relatively strong.Conclusion:For the first time,endophytic fungi from D.officinale were used to degrade its polysaccharides,yielding four potential strains capable of degrading DOP.Strain D-25 exhibited the best degradation effect and the highest polysaccharide yield.关键词
铁皮石斛多糖/内生真菌/产物组分分析/降解/筛选Key words
Dendrobium officinale polysaccharide/endophytic fungi/analysis of product components/degradation/screening分类
医药卫生引用本文复制引用
席星雨,郭兴利,胡江苗,康前进..降解铁皮石斛多糖的内生真菌筛选及产物组分分析[J].中国现代中药,2025,27(7):1283-1290,8.基金项目
云南省科技厅科技计划项目(202401AS070075) （202401AS070075）
