遵义医科大学学报2025，Vol.48Issue(7)：674-681,8.
BMP2过表达对骨髓间充质干细胞兔前交叉韧带重建腱-骨愈合的影响
The effect of BMP2 overexpression on tendon-bone healing in rabbit anterior cruciate ligament reconstruction using bone marrow mesenchymal stem cells
摘要
Abstract
Objective To investigate the role of overexpression of bone morphogenetic protein 2(BMP2)in ten-don-bone healing during anterior cruciate ligament(ACL)reconstruction in New Zealand rabbits,using bone marrow mesenchymal stem cells(BMSCs).Methods An intra-articular incision adjacent to the medial patella was performed to establish a rabbit model for ACL reconstruction.Seventy-two New Zealand white rabbits were randomly divided into four groups:sham-operation group,model group,BMSCNC group(implanted with BMSCs in fibrin glue infected with negative control vector),and BMSCBMP2 group(implanted with BMSCs in fibrin glue in-fected with BMP2-overexpressing lentivirus),with 18 rabbits in each group.Histological analysis(HE staining and Masson staining),Micro-CT,and biomechanical testing were used to evaluate tendon-bone healing,bone for-mation,and biomechanical properties after ACL reconstruction.Results In the BMSCNC and BMSCBMP2 groups,it could be seen that mature cartilage zones were gradually fused with adjacent tendons and bones.Tendon-bone tunnels with dense fibrous tissue and a small amount of osseous tissue closely connected were observed,along with prominent fibroblasts and collagen fibers.Compared to the sham-operation group,the model group exhibited se-vere damage to cartilage and bone structures,with decreased bone mineral density,bone volume fraction(BV/TV),and protein expression of alkaline phosphatase(ALP),osteocalcin(OCN),and osteopontin(OPN)at the tendon-bone interface(P＜0.05).Additionally,the maximum load and stiffness of the tendon graft complex were reduced(P＜0.05).In comparison to the model group,both the BMSCNC group and the BMSCBMP2 group showed improvement in cartilage and bone structure damage,with new bone formation observed around the graft.There was an increase in bone mineral density,BV/TV,and protein expression of ALP,OCN,and OPN at the tendon-bone interface,along with an elevation in the maximum load and stiffness of the tendon graft complex(P＜0.05).Moreover,the BMSCBMP2 group demonstrated higher values than the BMSCNC group(P＜0.05).Conclusion BMSCs with BMP2 overexpression can accelerate tendon-bone interface bone healing in the ACL reconstruction New Zealand rabbits model.关键词
前交叉韧带/骨形态发生蛋白2/腱-骨愈合/骨髓间充质干细胞Key words
anterior cruciate ligament/bone morphogenetic protein 2/tendon-bone healing/bone marrow mes-enchymal stem cell分类
医药卫生引用本文复制引用
张昕悦,尹帅,刘国强,李永犇,张海峰,宋效雷,刘媛媛..BMP2过表达对骨髓间充质干细胞兔前交叉韧带重建腱-骨愈合的影响[J].遵义医科大学学报,2025,48(7):674-681,8.基金项目
河北省卫生健康委2022年度医学科学研究课题计划项目(NO:20220038). （NO:20220038）
