遵义医科大学学报2025，Vol.48Issue(7)：726-731,6.
2021-2023年山东省烟台市HIV-1抗体不确定样本首次检测及随访结果分析
Analysis of the first detection and follow-up results of samples with HIV-1 anti-body indeterminate in Yantai,Shandong Province,2021-2023
摘要
Abstract
Objective To explore the value of human immunodeficiency virus(HIV)antibody screening test re-sults,western blot(WB)band type and viral load(VL)in the diagnosis of HIV-1 antibody indeterminate sam-ples.Methods The subjects with antibody reactivity in screening tests[rapid test(RT)and enzyme-linked im-munosorbent assay(ELISA)]conducted in HIV confirmatory laboratory in Yantai of Shandong Province from 2021 to 2023 were collected for WB.A total of 72 subjects with HIV-1 indeterminate antibody were followed-up and HIV-1 VL test was performed.Results The detection rate of VL was 85.19％in 54 samples which were de-tected positive by both RT and ELISA.Statistical analysis revealed that the differences in the detection rate of VL among the four groups according to sample/cut-off(S/CO)value of 72 HIV-1 antibody uncertain samples were statistically significant(P＜0.05).Among these groups,the detection rate of VL was highest when S/CO≥12.Gp160 p24(28/72)and gp160(23/72)had the most WB bands in the HIV-1 antibody indeterminate samples.The positive rate of VL among the cases with WB bands on env+pol,env+pol+gag,env+gag was 100％,100％and 93.94％,respectively.Five cases with VL＞5 000 copies/ml was detected among the 19 cases lost to follow-up.Conclusion The VL test can accurately identify the infection status of individuals with HIV-1 indeter-minate antibody and shorten the diagnosis time of HIV infection.The results of screening test,S/CO and WB bands can provide a reference for the diagnosis of HIV-1 infection to a certain extent.关键词
人类免疫缺陷病毒/抗体不确定/酶联免疫吸附试验/免疫印迹试验/病毒载量检测Key words
human immunodeficiency virus/antibody indeterminate/enzyme-linked immunosorbent assay/western blot/viral load test分类
医药卫生引用本文复制引用
张艺,孙振璐,杨柳,刘娟,冷启艳,丁学斌,杨建..2021-2023年山东省烟台市HIV-1抗体不确定样本首次检测及随访结果分析[J].遵义医科大学学报,2025,48(7):726-731,6.基金项目
山东省学生卫生协会科研资助项目(NO:SDWS2024088). （NO:SDWS2024088）
