中药药理与临床2025，Vol.41Issue(5)：49-55,7.
青钱柳介导AKT/mTOR/SREBP-1c信号通路缓解糖尿病合并非酒精性脂肪肝
Mechanism of Cyclocarya Paliurus in Alleviating Diabetes Mellitus Combined with Nonalcoholic Fatty Liver Disease via Mediating AKT/mTOR/SREBP-1c Signaling Pathway
摘要
Abstract
Objective:To explore the effect and mechanism of Cyclocarya paliurus on hepatic lipid metabolism in rats with diabetes mellitus and nonalcoholic fatty liver disease based on the AKt/mTOR/SREBP-1c signaling pathway.Methods:Sprague-Dawley(SD)rats were randomly divided into a normal control group,a model control group,a positive control group(10 mg/kg pioglitazone),and three Cyclocarya paliurus groups(0.45,0.90,and 1.80 mg/kg).After one month of continuous gavage,tail vein blood samples were collected to assess fasting blood sugar(FBS)levels in rats.The levels of alanine aminotransferase(ALT),aspartate aminotransferase(AST),triglyceride(TG),and total cholesterol(TC)were measured using an automatic biochemical analyzer,and the contents of malondialdehyde(MDA),glutathione(GSH),and superoxide dismutase(SOD)in liver tissue were determined using kits.Histopathological analysis of liver morphology was performed u-sing H&E staining,and hepatic lipid accumulation was observed using oil red O staining.The microstructure of liver tissue was observed via transmission electron microscopy.Additionally,mRNA expression levels of Cd36 and Atgl in rat liver were quantified using qPCR,and the protein expressions of AKT,p-AKt,mTOR,p-mTOR,SREBP-1c,LC3,and P62 were assessed by Western blot.Results:Compared with the normal control group,the model control group exhibited significantly up-regulated FBS,TG,TC,ALT,AST,MDA,and Cd36 mRNA expressions(P<0.01),increased p-AKt,p-mTOR,P62,and SREBP-1c protein expressions(P<0.01),and significantly reduced levels of LC3II/LC3I(P<0.01).In the model control group,the rat liver structure was disordered,with pronounced steatosis.Compared with the model control group,the 1.80 mg/kg Cyclocarya paliurus group displayed significantly down-regulated MDA,p-Akt,p-mTOR,P62,and SREBP-1c protein expressions,reduced Cd36 mRNA expression(P<0.01),elevated LC3II/LC3I,and significantly up-regulated Atgl mRNA expression(P<0.01).Conclusion:Cyclocarya paliurus alleviates hepatic lipid metabolism disorder and oxidative stress in rats with diabetes mellitus com-bined with nonalcoholic fatty liver disease.The underlying mechanism may involve the inhibition of AKt/mTOR/SREBP-1c signaling path-way.关键词
青钱柳/糖尿病/非酒精性脂肪肝/蛋白激酶/雷帕霉素蛋白/胆固醇调节元件结合蛋白-1c通路Key words
Cyclocarya paliurus/Diabetes mellitus/Nonalcoholic fatty liver disease/AKt/mTOR/SREBP-1c pathway引用本文复制引用
刘艺璇,吴昊洋,张亚男,吴琴,陈静,李定祥,邓奕辉..青钱柳介导AKT/mTOR/SREBP-1c信号通路缓解糖尿病合并非酒精性脂肪肝[J].中药药理与临床,2025,41(5):49-55,7.基金项目
湖南省科技创新计划资助(编号:2020RC4050) （编号:2020RC4050）
湖南省卫生健康委科研课题(编号:D202303067824) （编号:D202303067824）
长沙市自然基金(编号:kq2208188) （编号:kq2208188）
湖南省研究生科研创新项目(编号:CX20220787). （编号:CX20220787）
