中药药理与临床2025，Vol.41Issue(5)：55-60,6.
枳壳潜在燥性成分3,5,6,7,8,3',4'-七甲氧基黄酮对正常小鼠津液及胃水通道蛋白的影响研究
Effects of Potential Drying Component 3,5,6,7,8,3′,4′-heptamethoxyflavone in AURANTII FRUCTUS on Aquaporin in Stomach and Body Fluids in Normal Mice
摘要
Abstract
Objective:To study the effects of 3,5,6,7,8,3′,4′-heptamethoxyflavone(HMF),a potential drying component of AURANTII FRUC-TUS,on aquaporin(AQP)in stomach and body fluids in normal mice and to establish a new strategy for research on the scientific connotation of drug properties.Methods:Male C57BL/6 mice were randomly divided into a normal group,a 4 g/kg AURANTII FRUCTUS group,and 0.4 mg/kg,0.8 mg/kg,and 1.6 mg/kg HMF groups.The following indicators of each group were determined:24 h drinking water intake,food intake,2 h defecation rate and fecal water content,salivary secretion,small intestinal propulsion distance,and water content of gastric and sub-mandibular gland tissue.Hematoxylin-eosin(HE)staining was used to observe and score the pathological morphology of gastric tissue.The expression of AQP3,AQP5,and AQP7 extracted from gastric tissue was assessed by immunohistochemistry(IHC).Results:Compared with the normal group,the 0.8 mg/kg and 1.6 mg/kg HMF groups showed significantly increased drinking water intake(P<0.01),and the 0.8 mg/kg HMF group and the 4 g/kg AURANTII FRUCTUS group had significantly increased food intake(P<0.01).Additionally,the sali-vary secretion,2 h defecation rate,and fecal water content were significantly reduced in the 0.4 mg/kg,0.8 mg/kg,and 1.6 mg/kg HMF groups and the 4 g/kg AURANTII FRUCTUS group(P<0.01).The small intestinal propulsion distance was significantly reduced in the 0.8 mg/kg HMF group(P<0.05),while it exhibited highly significant reductions in the 0.4 mg/kg and 1.6 mg/kg HMF groups and the 4 g/kg AURANTII FRUCTUS group(P<0.01).The water content of submandibular gland tissue in all the medication groups was significant-ly reduced(P<0.05 or P<0.01),which was in the same trend of salivary secretion.Pathological damages(edema,inflammation,necrosis,etc.)in the gastric tissue were observed,with the damage scores of gastric tissue in mice of all the HMF treatment groups significantly in-creasing(P<0.01).The AQP3 expression was significantly increased in the 0.8 mg/kg HMF group(P<0.05),and the AQP5 expression in gastric tissue was significantly increased in the 4 g/kg AURANTII FRUCTUS group and the 0.8 mg/kg and 1.6 mg/kg HMF groups(P<0.01),while the AQP7 expression in gastric tissue was significantly increased in the 0.8 mg/kg and 1.6 mg/kg HMF groups(P<0.01).Conclusion:The drying property of AURANTII FRUCTUS and HMF can affect the secretion of submandibular gland and the metabolism of gastric fluid in mice,and the mechanism may be related to up-regulating the expression of AQP3,AQP5,and AQP7 in gastric tissue.关键词
枳壳/3,5,6,7,8,3',4'-七甲氧基黄酮/燥性/水通道蛋白/小肠推进率/口干/便秘/颌下腺分泌失常Key words
AURANTII FRUCTUS/Drying property/3,5,6,7,8,3′,4′-Heptamethoxyflavone/Aquaporin(AQP)/Intestinal propulsive rate/Thirst/Constipation/Submandibular gland dysfunction引用本文复制引用
辛力,温佳文,谢亚婷,龚文慧,郭静英,郑郁清,平欲晖,张金莲..枳壳潜在燥性成分3,5,6,7,8,3',4'-七甲氧基黄酮对正常小鼠津液及胃水通道蛋白的影响研究[J].中药药理与临床,2025,41(5):55-60,6.基金项目
国家自然科学基金(编号:82060724、81560651) （编号:82060724、81560651）
大学生创新创业计划项目(编号:202310412027、S202310412061、202210412288) （编号:202310412027、S202310412061、202210412288）
江西省标准化项目(编号:2023A19、2021B01、2021B02) （编号:2023A19、2021B01、2021B02）
江西省高水平本科教学团队建设项目(编号:2022114). （编号:2022114）
