中药药理与临床2025，Vol.41Issue(6)：30-35,6.
肝宝胶囊对代谢功能障碍相关脂肪性肝炎(MASH)治疗作用机理研究
Therapeutic Mechanism of Ganbao Capsules in Treating Metabolic Dysfunction-Associated Steatohepatitis(MASH)
摘要
Abstract
Objective:The morbidity of metabolic dysfunction-associated steatohepatitis(MASH)is increasing year by year,while no effective thera-peutic drug is available now.Methods:Neonatal mice were subcutaneously injected with monosodium glutamate(MSG,5 g/kg)to induce o-besity and then fed a methionine-choline-deficient(MCD)diet to induce MASH.Meanwhile,a normal control group was designed.After 4 weeks,MASH mice were randomly grouped as follows:model control,Ganbao(肝宝)Capsules(2,4,and 8 g/kg),and obeticholic acid(30 mg/kg).Mice were administrated with corresponding drugs by gavage,once daily for four consecutive weeks.Serum and liver tissue samples were collected after the final administration following a 14-h fasting period(water permitted)for subsequent measurement of relevant indices.Results:Compared with the normal control group,obese mice being fed a MCD diet for 8 weeks showed elevated serum levels of transaminase and total bile acid(P<0.01),declined serum levels of lipids,and raised levels of triglycerides,total cholesterol,and hydroxypro-line in the liver tissue(P<0.01).Compared with the model control group,Ganbao Capsules dose-dependently ameliorated liver dysfunction,steatosis,necrosis,and fibrosis,down-regulated the protein levels of low-density lipoprotein receptor(LDLR)and ATP-binding cassette sub-family G member 5(ABCG5)and the mRNA levels of fatty acid synthase(Fasn)and 3-hydroxy-3-methylglutaryl-CoA reductase(Hmgcr)(P<0.05),and up-regulated the mRNA levels of ATP-binding cassette transporter A1(Abca1)and liver X receptor α(Lxrα)(P<0.05).Conclusion:Ganbao Capsules demonstrated significant therapeutic effects against MCD diet-induced MASH.The underlying mechanisms may involve suppression of SREBP2 and FASN expression to inhibit triglyceride synthesis,as well as inhibition of the LXRα/HMGCR,LXRα/LD-LR,and SREBP2/LDLR pathways to reduce cholesterol synthesis,transport,and accumulation in the liver.关键词
肝宝胶囊/代谢功能障碍相关脂肪性肝炎/蛋氨酸-胆碱缺乏饮食/谷氨酸钠/肥胖小鼠Key words
Ganbao Capsules/Metabolic dysfunction-associated steatohepatitis/Methionine-choline-deficient diet/Monosodium glutamate/Obese mice引用本文复制引用
邓江婷,曾青山,王淳,杜仕静,熊静悦,李晓媛,谭婉莹,谭正怀..肝宝胶囊对代谢功能障碍相关脂肪性肝炎(MASH)治疗作用机理研究[J].中药药理与临床,2025,41(6):30-35,6.基金项目
四川省中医药管理局科技研究专项(编号:2023zd010) （编号:2023zd010）
四川省科技厅中央引领地方发展专项(编号:2021ZYD0088). （编号:2021ZYD0088）
