中华中医药学刊2025，Vol.43Issue(7)：1-5,后插1-后插2,7.DOI:10.13193/j.issn.1673-7717.2025.07.001
基于甘味及甜味受体探讨玉竹皂苷降血糖作用机制
Exploration on Hypoglycemic Mechanism of Yuzhu(Polygonatum odoratumin)Saponin Based on Sweet Taste and Sweet Receptors
摘要
Abstract
Objective To define"sweetness"in traditional Chinese medicine based on sweet taste receptors and explore the hypoglycemic mechanism of Yuzhu(Polygonatum odoratumin)saponin.Methods The diabetic model was established by strepto-zocin(STZ)method.After successfully modeling,the mice were randomly divided into model group,metformin group,low dose of Yuzhu(Polygonatum odoratumin)saponin group,high dose of Yuzhu(Polygonatum odoratumin)saponin group,low dose of Yuzhu(Polygonatum odoratumin)saponin+Lac(Lactisole)group and high dose of Yuzhu(Polygonatum odoratumin)saponin+Lac group.The mice without modeling were taken as the normal group.Fasting blood glucose and glucose tolerance were tested 7 weeks after administration of the drug.After 8 weeks of drug administration,the levels of total cholesterol(TC),triglyceride(TG),low-density lipoprotein cholesterol(LDL-C)and high-density lipoprotein cholesterol(HDL-C)were determined by biochemical kits.The morphology of pancreas and liver tissues were observed by hematoxylin-eosin staining(HE),and the con-tents of glucagon-like peptide-1(GLP-1)and insulin were determined by enzyme linked immunosorbent assay(ELISA).HuTu-80 was used as a cellular model,and the contents of GLP-1 and Cyclic adenosine monophosphate(cAMP)were detec-ted by ELISA.The intensity of Ca2+fluorescence was detected by laser confocal.Results Yuzhu(Polygonatum odoratumin)sap-onin significantly reduced the levels of fasting blood glucose,TC,TG and LDL-C in diabetic mice(P＜0.05).Yuzhu(Polygo-natum odoratumin)saponin increased the levels of HDL-C,GLP-1 and insulin(P＜0.05),and improved the morphology of the pancreas and liver tissues.Yuzhu(Polygonatum odoratumin)saponin significantly increased the contents of cAMP,Ca2+and GLP-1 after their action on cells(P＜0.05).However,the action of Yuzhu(Polygonatum odoratumin)saponin could be signifi-cantly inhibited by the sweet taste receptor inhibitor Lactisole(P＜0.05).Conclusion Yuzhu(Polygonatum odoratumin)saponin agitates sweet taste receptors due to the sweet taste,which in turn promotes GLP-1 secretion and exerts hypoglycemic effects.关键词
甘味/甜味受体/玉竹/胰高血糖素样肽-1Key words
sweet taste/sweet receptor/Yuzhu(Polygonatum odoratumin)/glucagon-like peptide-1分类
医药卫生引用本文复制引用
杨华生,肖音,李婷,段亚文,吴璐..基于甘味及甜味受体探讨玉竹皂苷降血糖作用机制[J].中华中医药学刊,2025,43(7):1-5,后插1-后插2,7.基金项目
国家自然科学基金项目(81960724) （81960724）
江西省自然科学基金面上项目(20232BAB206171) （20232BAB206171）
江西省大学生创新创业训练计划项目(S202310412101) （S202310412101）
