中华中医药学刊2025，Vol.43Issue(7)：21-25,后插6-后插7,7.DOI:10.13193/j.issn.1673-7717.2025.07.005
甘露消毒丹对流感病毒感染"升清降浊"的机制研究
"Ascending The Clear and Descending The Turbid"Mechanism of Ganlu Xiaodu Micropill(甘露消毒丹)on Ⅳ Infection
摘要
Abstract
Objective The study investigated the molecular mechanism of"ascending the clear and descending the turbid"effect of Ganlu Xiaodu Micropill(甘露消毒丹,GXM)on retinoicacid-inducible gene 1(RIG-1)/mitochondrial antiviral sig-naling protein(MAVS)/nuclear factor-κB(NF-κB)pathway in influenza virus(Ⅳ)-infected mice.Methods Ninety-six SPF-grade BALB/c mice were randomly divided into normal,model,GXM and Oseltamivir groups.Except for the mice in the normal group,the Ⅳ infection mode in the other groups was set up and sampled on day 7 of modelling after the mouse abscission experiment.HE staining was used to observe pathological changes in lung tissues.The pathological changes in organelles of mouse lung tissue cells were observed under electron microscope.The mRNA expressions of nuclear factor kappa-B p65(NF-κB p65),MAVS,RIG-1 and lactate dehydrogenase A(LDHA)were detected in mouse lung tissues by qPCR method.Western blot method was used to determine the protein contents of MAVS,NF-κB and RIG-1 in mouse lung tissues and serum levels of interleukin 6(IL-6),LDH,Interferon-β(IFN-β)and interleukin-10(IL-10)were detected in mouse serum by ELISA method.Results Compared with those in the normal group,after Ⅳ infection,the alveolar structure of mice was severely dam-aged,with the presence of a large number of inflammatory cell infiltration and capillary dilatation;the ultrastructure of lung tissue was damaged,the alveolar structure was broken,the number of organelles was reduced,and part of the outer membrane and cristae of mitochondria were reduced or disappeared;the total distance and the average speed of walking of mice in all the other groups were significantly reduced(P＜0.05).The pulmonary tissue of mice had a significant decrease in the expressions of RIG-1,NF-κB p65,MAVS,LDHA mRNA and protein in the lung tissues of mice were significantly elevated;the serum levels of IL-6,IFN-β and LDH in mice were elevated,and the serum IL-10 level was decreased(P＜0.05).Compared with those in the model group,the total distance and the average speed of walking were significantly increased in the GXM group,the average speed of walking was significantly higher in the Oseltamivir group,and the average speed of mice in the Oseltamivir group was signifi-cantly lower than that in the GXM group(P＜0.05).The expressions of RIG-1,NF-κB p65,MAVS,LDHA mRNA and pro-tein were reduced,the serum levels of IL-6,IFN-β and LDH were reduced,and the serum levels of IL-10 were increased(P＜0.05),and the trends of changes were consistent between the two treatment groups after administration of the drug,in which the expression of RIG-Ⅰ mRNA in lung tissues of the GXM group was decreased significantly,and the improvement of serum lev-els of IL-6 and IFN-β in mice of the Oseltamivir group was obvious(P＜0.05).Conclusion GXM regulates the expressions of inflammatory factors and regulates energy metabolism in Ⅳ infection,possibly through the RIG-Ⅰ/MAVS/NF-κB pathway,which regulates the"ascending the clear and descending the turbid"effects of Ⅳ infection.关键词
流感病毒/甘露消毒丹/升清降浊/能量代谢/RIG-1/MAVS/NF-κBKey words
influenza virus/Ganlu Xiaodu Micropill(甘露消毒丹)/ascending the clear and descending the turbid/energy metabolism/RIG-1/MAVS/NF-κB分类
医药卫生引用本文复制引用
刘光华,张文涵,吴振起,刘玉婷,曲根生,赵健翔,赵炟..甘露消毒丹对流感病毒感染"升清降浊"的机制研究[J].中华中医药学刊,2025,43(7):21-25,后插6-后插7,7.基金项目
国家自然科学基金青年科学基金项目(81403293) （81403293）
国家中医药管理局第五批全国中医临床优秀人才项目(国中医药人教函[2022]239号) （国中医药人教函[2022]239号）
辽宁省教育厅重点攻关项目(LJKZZ20220104) （LJKZZ20220104）
辽宁中医药大学自然科学类重点项目(2021LZY039) （2021LZY039）
