中华中医药学刊2025，Vol.43Issue(7)：29-34,后插9-后插15,13.DOI:10.13193/j.issn.1673-7717.2025.07.007
联合微生物组学和代谢组学研究党参水提物调节衰老小鼠肠道屏障和菌群代谢的作用机制
Combining Microbiomics and Metabolomics to Study Mechanism of Dangshen(Codonopsis pilosula)Water Extract in Regulating Intestinal Barrier and Microbial Metabolism in Aging Mice
摘要
Abstract
Objective To reveal the protective effects of Dangshen(Codonopsis pilosula)water extract on intestinal barrier and microbial metabolism in aging model mice.Methods Fifty specific pathogen free(SPF)grade 8-week-old C57BL/6 mice were randomly divided into 5 groups to construct a D-galactose mouse aging model,and different doses of Dangshen(Codonopsis pilo-sula)water extract were used for intervention.Biochemical analysis was used to detect the levels of superoxide dismutase(SOD),malondialdehyde(MDA)and lipid peroxidation(LPO)in mouse serum.Haematoxylin Eosin(HE)staining was used to observe pathological changes in mouse intestinal tissue.Immunohistochemical staining was used to observe the expressions of Oc-cludin and Claudin 1 proteins in mouse intestinal tissue.The ultrastructure of mouse intestinal tissue cells was observed by using transmission electron microscopy.The combination of 16S rDNA sequencing and metabolomics analysis was used to detect the changes in gut microbiota and metabolites in mice after aging and Dangshen(Codonopsis pilosula)intervention,while joint analy-sis was conducted through bioinformatics.Results Compared with those of the normal control group,the weight and food intake of aging mice decreased(P＜0.05),and the weight and food intake of the intervention group with Dangshen(Codonopsis pilosula)significantly increased compared to those of the aging group(P＜0.05).Compared with those in the normal control group,the se-rum SOD in the aging group decreased(P＜0.05),while the levels of MDA and LPO increased(P＜0.05).The intervention group with Dangshen(Codonopsis pilosula)significantly reversed the level change of SOD,MDA and LPO(P＜0.05).The intes-tinal tissue structure of aging model mice was significantly damaged,and the expressions of Occludin and Claudin 1 proteins de-creased significantly compared to those of the normal controls(P＜0.05).After high-dose intervention with Dangshen(Codo-nopsis pilosula),there was a significant improvement in intestinal tissue damage,and the expressions of Occludin and Claudin 1 proteins increased significantly(P＜0.05),and the ultrastructure of mouse intestinal cells tended to be normal.During the aging process of mice,the ratio of Bacteroidota to Firmicutes was decreased,after high-dose intervention with Dangshen(Codonopsis pi-losula),the ratio was increased.During this process,the main differential metabolites were lipids and lipid molecules,organic acids and their derivatives.Metabolic pathways included galactose metabolism,glyceride metabolism and drug metabolism-cyto-chrome P450.Omics association analysis showed that Paraacteroides,Lactobacillus and Lachnochlostrium were closely related to lipids and lipid molecules.Conclusion Dangshen(Codonopsis pilosula)water extract has a significant protective effect on the in-testinal tissue structure and function of aging mice.By regulating the intestinal microbiota structure and its metabolites,it im-proves the intestinal barrier function and resists aging in the body.关键词
代谢组学/16S rDNA/衰老/肠道菌群/党参Key words
metabolomics/16S rDNA/aging/gut microbiota/Dangshen(Codonopsis pilosula)分类
医药卫生引用本文复制引用
王晶,王振娟,康甲超,韩乐,柳立军,吴萍民,段永强..联合微生物组学和代谢组学研究党参水提物调节衰老小鼠肠道屏障和菌群代谢的作用机制[J].中华中医药学刊,2025,43(7):29-34,后插9-后插15,13.基金项目
国家自然科学基金项目(82060829) （82060829）
甘肃省自然科学基金项目(23JRRA1720) （23JRRA1720）
甘肃中医药大学研究生创新创业基金项目(2023CX) （2023CX）
