安徽农业科学2025，Vol.53Issue(15)：32-36,5.DOI:10.3969/j.issn.0517-6611.2025.15.008
多壁碳纳米管生根培养基对草莓脱毒苗生长的影响
Effect of Multi-walled Carbon Nanotube Rooting Medium on the Growth of Strawberry Virus-free Seedlings
摘要
Abstract
[Objective]To establish a strawberry tissue culture system based on carbon nanotube medium.[Method]This study used'Hongy-an'strawberry as the experimental material to explore the influence of multi-walled carbon nanotube rooting medium with different concentra-tions(25,50 and 100 mg/L)on the growth of strawberry tissue culture seedlings,and observed the growth of adventitious roots.[Result]The results showed that adding multi-walled carbon nanotubes to the strawberry rooting culture medium significantly improved the callus induction rate.Compared with the control group,50 mg/L multi-walled carbon nanotube rooting medium had the best effect,increasing the height of vi-rus-free seedlings by 27.1%,the total root length by 83.4%,the total root volume by 163.8%,and the number of root tips by 70.1%.Mi-crostructural observations revealed that the multi-walled carbon nanotubes were absorbed on the surface of the adventitious roots and did not ag-glomerate inside them.The survival rate of transplanting after acclimatization was above 95%,and the young seedlings grew well.[Conclu-sion]In this study,a multi-walled carbon nanotube rooting medium(50 mg/L)suitable for strawberry tissue culture was selected to provide technical support for the application of carbon nanomaterials in strawberry propagation.关键词
'红颜'草莓/碳纳米管/组织培养/微观结构/不定根Key words
'Hongyan'strawberry/Carbon nanotube/Tissue culture/Microstructure/Adventitious root分类
农业科技引用本文复制引用
曹凡,金雨晴,谷青青,王莹,陈燕,徐秋霞,吴玲,黄欣,李玉娟..多壁碳纳米管生根培养基对草莓脱毒苗生长的影响[J].安徽农业科学,2025,53(15):32-36,5.基金项目
江苏省农业科学院探索性颠覆性创新计划项目(ZX(21)1231) （ZX(21）
江苏省农业科技自主创新资金项目(CX(23)3133) （CX(23）
江苏现代农业(草莓)产业技术体系海门推广示范基地项目(JATS[2023]145) （草莓）
江苏沿江地区农业科学研究所博士科技基金项目(YJBS[2021]004). （YJBS[2021]004）
