草业科学2025，Vol.42Issue(7)：1632-1641,10.DOI:10.11829/j.issn.1001-0629.2025-0052
Cd胁迫下硝态氮肥联合乙二胺二琥珀酸对三叶鬼针草生长和活性氧代谢的影响
Effects of nitrate nitrogen fertilizer combined with ethylenediamine disuccinic acid on the growth and active oxygen metabolism of Bidens pilosa under Cd stress
摘要
Abstract
To explore the physiological,biochemical,and antioxidant mechanisms of Bidens pilosa in response to cadmium(Cd)stress under the combined action of nitrate nitrogen fertilizer and ethylenediamine disuccinic acid(EDDS),an indoor pot experiment was conducted to study the effects of 75 mg·kg-1(as nitrogen)nitrate nitrogen fertilizer combined with 1 mmol·L-1 EDDS on the growth and active oxygen metabolism of Bidens pilosa under 15 mg·kg-1 Cd stress.Under Cd stress,plant height,root length,chlorophyll a(Chl a),chlorophyll b(Chl b),total chlorophyll(Chl),and soluble protein(SP)content of B.pilosa reached maximum values with the addition of nitrate nitrogen fertilizer alone.The addition of EDDS alone resulted in the highest soluble sugar(SS)content.Under Cd stress,the combination of nitrate-nitrogen fertilizer and EDDS increased the plant height,Chl a,and Chl content of B.pilosa by 24.29％,51.06％,and 21.09％,respectively,compared with Cd treatment alone.It significantly enhanced the activities of ascorbate peroxidase(APX),catalase(CAT),peroxidase(POD),and superoxide dismutase(SOD)by 37.04％,22.92％,18.31％,and 197.81％,respectively,compared to the Cd treatment alone,reaching maximum values among all treatments.Additionally,the SP and SS contents were maximized,whereas the malondialdehyde(MDA)content and relative electrical conductivity(REC)decreased significantly by 45.77％and 21.25％,respectively,compared to the Cd treatment alone.In summary,75 mg·kg-1 nitrate nitrogen fertilizer combined with 1 mmol·L-1 EDDS can improve the tolerance of B.pilosa to Cd stress.Combined remediation technologies can promote the growth of aboveground parts and maintain the normal accumulation of biomass by increasing the Chl a and Chl content in plants.By enhancing the antioxidant enzyme activities and increasing osmotic adjustment content,the membrane lipid peroxidation damage caused by Cd was alleviated,and the cell membrane was maintained.关键词
镉胁迫/植物修复/螯合剂/抗氧化机制/三叶鬼针草/主成分分析Key words
cadmium stress/phytoremediation/chelating agent/antioxidant mechanism/Bidens pilosa/principal component analysis引用本文复制引用
陈银萍,曹渤,卢誉之,孙勇,李倩,袁巧玲..Cd胁迫下硝态氮肥联合乙二胺二琥珀酸对三叶鬼针草生长和活性氧代谢的影响[J].草业科学,2025,42(7):1632-1641,10.基金项目
国家自然科学基金项目(32260316) （32260316）
自然资源部黄河上游战略性矿产资源重点实验室2023年度开放基金项目(YSMRKF202306) （YSMRKF202306）
甘肃省科技计划项目(24JRRA1171) （24JRRA1171）
