重庆医学2025，Vol.54Issue(7)：1521-1526,1533,7.DOI:10.3969/j.issn.1671-8348.2025.07.001
维生素D对肥胖大鼠焦虑抑郁行为的影响及其相关海马体炎症机制的研究
Effect of vitamin D on anxiety and depressive behaviors and related mechanisms of hippocampal inflammation in obese rats
摘要
Abstract
Objective To explore the effects of vitamin D on anxiety and depression behavior and the mechanism of hippocampal inflammation in obese rats.Methods Male Wistar rats were randomly divided into 4 groups(n=12):control group(group C),obesity group(group F),low-dose vitamin D group(L-VD group),and high-dose vitamin D group(H-VD group).Group C was fed a standard diet,and the other three groups were fed a high-fat diet.After successful establishment of the rat obesity model,L-VD group and H-VD group were intraperitoneally injected with 10 μg·kg-1·d-1 and 20 μg·kg-1·d-1 vitamin D,respective-ly,and groups C and F were injected with the same volume of normal saline;relevant indexes were detected af-ter continued feeding for 6 weeks.The sucrose preference test and open field test were used to study the be-havior of rats in each group.The histopathological changes of hippocampal tissue were observed by HE stai-ning.The expression levels of TNF-α and NF-κB protein in hippocampal tissue were detected by Western blot.Results After 6 weeks of intervention,compared with group C,the serum 25(OH)D concentration,sugar-wa-ter preference rate,total distance of open field movement,number of entries into the central area,and duration of stay in the central area were significantly decreased in group F rats.The expression levels of TNF-α and NF-κB proteins in hippocampal tissues were significantly increased(P<0.05).Compared with group F,serum 25(OH)D concentration and sugar-water preference rate were significantly increased in L-VD and H-VD groups.Anxiety and depression-like behaviors in the open field were markedly improved.TNF-α and NF-κB protein levels were significantly decreased(P<0.05).Statistically significant differences were observed in all aforementioned indicators between the groups(P<0.05).HE staining revealed looser arrangement of hipp-ocampal neurons,with extensive neuronal degeneration in group F.In contrast,L-VD and H-VD groups exhib-ited tighter and more regular neuronal arrangements,with only mild edema and cytoplasmic vacuolation in some cells.No significant neuronal degeneration was observed in the H-VD group.Conclusion Reduced vita-min D levels in obese rats exacerbate anxiety-depression-like behaviors,potentially through activation of hipp-ocampal inflammatory factors.关键词
维生素D/肥胖/抑郁/海马体/炎症Key words
Vitamin D/obesity/depression/hippocampus/inflammation分类
医药卫生引用本文复制引用
董志萍,张喆,丛毓,刘艳..维生素D对肥胖大鼠焦虑抑郁行为的影响及其相关海马体炎症机制的研究[J].重庆医学,2025,54(7):1521-1526,1533,7.基金项目
国家自然科学基金项目(82173519) （82173519）
天津医科大学朱宪彝纪念医院科研基金项目(ZXY-YJJ2020-6). （ZXY-YJJ2020-6）
