福建农业学报2025，Vol.40Issue(4)：325-332,8.DOI:10.19303/j.issn.1008-0384.2025.04.001
青花菜乙烯响应因子基因BoiERF5的克隆及抗病功能分析
Cloning and Disease Resistance of BoiERF5
摘要
Abstract
[Objective]Bioinformatics of BoiERF5 was studied to understand the functions of ethylene response factors(ERFs)gene in response to gray mold and sclerotinia rot of a plant.[Method]The gene of ERFs in Brassica oleracea var.italica(BoiERF5)was cloned using PCR method,followed by sequence analysis and phylogenetic analysis using ProtParam,SMART,Clustal X and Mega 3.1.Expressions of the gene under Botrytis cinerea and Sclerotinia sclerotiorum infections were determined by qRT-PCR,and vectors constructed for the determinations of subcellular localization and disease resistance.[Result]The full-length of BoiERF5 was 891 bp without introns.It encoded 286 amino acids,contained one AP2/ERF domain,and was most homologous in the same phylogenetic tree with the proteins in B.cretica and B.rapa.After inoculating B.cinerea on broccoli leaves,the expression of BoiERF5 initially increased to peak in 36 h then decreased;while the S.sclerotiorum infection caused the gene to peak in 36 h/48 h.BoiERF5 located in nucleus,same as the predictions shown by online source.Overexpressed BoiERF5 significantly enhanced the resistance of Nicotiana benthamiana leaves to S.sclerotiorum and B.cinerea,which was accompanied by a significant increase in the expression of the pathogenesis-related NbPDF1.2 but not in that of NbPR1.[Conclusion]Expression of BoiERF5 could be induced by either B.cinerea or S.sclerotiorum.The overexpression significantly raised the resistance of N.benthamiana leaves to both pathogens.关键词
青花菜/乙烯响应因子/灰霉病/菌核病/抗病功能Key words
Brassica oleraceavar.italica/ethylene response factor/gray mold disease/sclerotinia disease/disease resistance分类
农业科技引用本文复制引用
曾浙芳,胡芯慈,周靓雯,李张群,郑德伟,张慧娟,蒋明..青花菜乙烯响应因子基因BoiERF5的克隆及抗病功能分析[J].福建农业学报,2025,40(4):325-332,8.基金项目
国家级大学生创新创业训练计划项目(2024103500031) （2024103500031）
台州市科技计划项目(22nya01) （22nya01）
