福建农业学报2025，Vol.40Issue(4)：333-340,8.DOI:10.19303/j.issn.1008-0384.2025.04.002
柠檬转录组SSR标记开发及遗传多样性分析
Transcriptome-based EST-SSR Search for Analysis on Lemon Genetic Diversity
摘要
Abstract
[Objective]EST-SSRs of lemon were searched based on transcriptome data to analyze the genetic diversity of the plant.[Method]SSR loci of lemon transcriptomes were screened using MISA to design primers by Primer 3.0.A clustering of 49 species was secured.[Result]Of the 40193 SSR loci identified,a distribution frequency of 35.67%and an average distribution distance of 1.39 kb were found in the sequences of 80.91%mono-,9.20%tri-,and 8.67%dinucleotide repeat types with the dominant motifs of A/T,AG/CT,and AAG/CTT,respectively.Twenty-four of the 4561 pairs of primers were selected for validity verification,and 13 by being polymorphic.The amplified 47 alleles had a mean effective value of 3.615 with the mean Ho,He,and PIC of 0.637,0.516,and 0.528,respectively.At the genetic distance of 0.40,the lemon cultivars were clustered into 5 categories,which were generally consistent with that obtained by the traditional morphological classification.[Conclusion]Thirteen pairs of EST-SSR primers were selected to classify 49 lemon specimens.The resulting clustering agreed with traditional taxonomy.The displayed rich genetic diversity rendered these cultivars an adequate germplasm collection for the development and utilization of lemon plants.关键词
柠檬/转录组/SSR重复类型/聚类分析Key words
lemon/transcriptome/EST-SSRs/cluster analysis分类
农业科技引用本文复制引用
余智城,何雪娇,陈振东,林秀香..柠檬转录组SSR标记开发及遗传多样性分析[J].福建农业学报,2025,40(4):333-340,8.基金项目
福建省科技计划公益类专项(2017R1028-1) （2017R1028-1）
漳州市自然科学基金项目(ZZ2020J15) （ZZ2020J15）
