福建农业学报2025，Vol.40Issue(4)：349-360,12.DOI:10.19303/j.issn.1008-0384.2025.04.004
盐生植物海雀稗不同种质对盐胁迫响应的转录组分析
Transcriptomes of Paspalum vaginatum Germplasms under Salt-stress
摘要
Abstract
[Objective]Transcriptomic techniques were applied to investigate the molecular mechanisms underlying the salt tolerance of Paspalum vaginatum.[Method]Physiological index changes in the salt-tolerant sealsle2000 and the salt-sensitive 17USA-45 under salt-stress were determined.RNA-seq technology was employed for transcriptome sequencing,and bioinformatics methods for analysis on differentially expressed genes(DEGs).[Result]Under salt-stress,the decreases in Pn,Gs,and SPAD as well as the increase in RLF of sealsle2000 were significantly lower than those of 17USA-45.Transcriptome analysis revealed that after 1 and 3 days of salt stress,6876 and 6017 DEGs were identified in sealsle2000,respectively,while 4457 and 5536 DEGs were identified in 17USA-45.An analysis suggested that the AP2 transcription factor gene family in P.vaginatum might play a pivotal role in the response to salt-stress.KEGG and GO enrichment analyses revealed that both genotypes were commonly enriched in pathways related to starch and sucrose metabolism,carbon metabolism,and photosynthetic components.Sealsle 2000 was specifically enriched in the pathways associated with brassinolide biosynthesis,salt-stress response,and mannitol response.In contrast,17USA-45 was enhanced in the pathways associated with phenylpropanoid biosynthesis and the NAD(P)H dehydrogenase complex.A qRT-PCR validation confirmed the high reliability of the RNA-seq data obtained in this study.[Conclusion]The salt-tolerant genotype of P.vaginatum was found to be specifically enriched in the brassinosteroid biosynthesis pathway,negative regulation of ethylene-activated signaling pathway,and response to mannitol.关键词
海雀稗/盐胁迫/转录组/差异表达基因Key words
Paspalum vaginatum/salt-stress/transcriptome/differentially expressed genes分类
农业科技引用本文复制引用
祝青,张叶,孙劝劝,胡旭,许廷晨,唐敏强,王志勇..盐生植物海雀稗不同种质对盐胁迫响应的转录组分析[J].福建农业学报,2025,40(4):349-360,12.基金项目
国家自然科学基金项目(32060409、32371782、32460358) （32060409、32371782、32460358）
海南省科技人才创新基金项目(KJRC2023C21) （KJRC2023C21）
海南省科技创新专项(ZDYF2023XDNY078) （ZDYF2023XDNY078）
海南大学南繁与高效热带农业协同创新中心项目(XTCX2022NYB08) （XTCX2022NYB08）
海南大学生态文明协同创新中心项目(XTCX2022STC10) （XTCX2022STC10）
