福建农业学报2025，Vol.40Issue(4)：406-414,9.DOI:10.19303/j.issn.1008-0384.2025.04.010
稻瘟病菌MoDock1和MoElmo1蛋白的功能分析
Pathogenic Functions of MoDock1 and MoElmo1 in Magnaporthe oryzae
摘要
Abstract
[Objective]Roles of the genes associated with MoDock1 and MoElmo1 proteins in Magnaporthe oryzae played in rice blast disease were studied.[Methods]Homologies of Dock180 and ELMO in M.oryzae were identified by bioinformatics method,and the coding genes knocked out to create mutants for determinations of phenotype and interactions between the two proteins by a co-immunoprecipitation test.[Results]MoDOCK1 and MoELMO1 were identified in M.oryzae and deleted to obtain the mutants that were almost identical in phenotype.Absence of the two proteins in the mutants significantly affected the conidial adhesion resulting in long bud tubes with septum and delayed the appressoria formation.Since the defective appressoria formation could be repaired by the addition of 8-Br-cAMP or IBMX,it suggested that MoDock1 and MoElmo1 might be involved in the cAMP-PKA signaling pathway.To test that hypothesis,onion epidermis was infected with the mutants to show a declined infectious ability,and on a rice plant,a lowered pathogenicity.Furthermore,the co-immunoprecipitation experiment confirmed the MoDock1-MoElmo1 interaction as well.[Conclusion]MoDock1 and MoElmo1 in M.oryzae provided the conidia adhesion and appressoria formation essential for the pathogenicity of the pathogen on rice.关键词
稻瘟病菌/Dock1/Elmo1/分生孢子粘着/附着胞Key words
Magnaporthe oryzae/Dock1/Elmo1/conidial adhesion/appressorium分类
农业科技引用本文复制引用
张承康,汪洋,黄欣,郭田龙,林伟..稻瘟病菌MoDock1和MoElmo1蛋白的功能分析[J].福建农业学报,2025,40(4):406-414,9.基金项目
福建省自然科学基金项目(2021J05254) （2021J05254）
宁德师范学院创新团队项目(2022T01) （2022T01）
