福建农业学报2025，Vol.40Issue(5)：441-448,8.DOI:10.19303/j.issn.1008-0384.2025.05.002
基于SSR标记分析108份金豆种质资源遗传多样性
SSR-based Analysis on Genetic Diversity of 108 Fortunella hindsi Germplasms
摘要
Abstract
[Objective]Genetic diversity of Fortunella hindsii germplasms was analyzed to facilitate resource utilization and breeding.[Methods]A collection of 108 F.hindsii germplasms was organized based on 13 agronomic traits and 10 pairs of SSR molecular markers.The characteristic genetic variations of the collection were analyzed according to the calculated variation coefficient,polymorphism information content(PIC),genetic differentiation index(Fst),and other parameters combined with the analysis of molecular variance(AMOVA)and clustering.[Results]The phenotypic differentiation of F.hindsii was rich with the variation coefficients ranging 10.51%-50.79%.There were 41 alleles detected in the SSR markers,averaging 4.1 alleles per locus.On average,the population showing a PIC of 0.459(locus B18 being the highest at 0.729),a Shannon's information index(I)of 0.945,an observed heterozygosity(Ho)of 0.324,and an expected heterozygosity(He)of 0.515 was genetically rich in diversity despite some degrees of inbreeding.It was clustered into 5 subpopulations without apparent correlation with the geographical distribution.The genetic variations revealed by AMOVA mainly originated within an individual germplasm at 53%and among individuals at 31%.The genetic differentiations among the subpopulations ranked from moderate to high with Fst=0.071-0.226.Among the subpopulations,GroupⅠtopped the rest with an Fst＞0.18.[Conclusion]The 108 F.hindsii germplasms demonstrated with high genetic diversity.The SSR markers applied for the analysis satisfactorily rendered the information needed for further studies on the resource conservation and breeding program.关键词
金豆/表型变异/简单序列重复(SSR)标记/遗传多样性Key words
Fortunella hindsi/phenotypic variation/SSR markers/genetic diversity分类
农业科技引用本文复制引用
卢小转,周淑芬,赵阳宇,王小安,田大刚..基于SSR标记分析108份金豆种质资源遗传多样性[J].福建农业学报,2025,40(5):441-448,8.基金项目
福建省科技计划公益类专项(2022R1028001) （2022R1028001）
科企合作项目(2022JDXM) （2022JDXM）
