福建农业学报2025，Vol.40Issue(5)：449-458,10.DOI:10.19303/j.issn.1008-0384.2025.05.003
抗感虫水稻受褐飞虱取食诱导的应激反应差异性分析
Stress Responses of Resistant and Susceptible Rice to Brown Planthopper Infestation
摘要
Abstract
[Objective]Stress responses of the insect-resistant and susceptible rice germplasms to brown planthopper(BPH)were compared to decipher the underlining mechanism for better control on one of the major pests on rice.[Method]BPHs were allowed to feed on the insect-resistant BP4 and insect-susceptible TN1 rice plants.Phenotypic changes on the plants of two contrasting varieties of rice were observed.In addition,contents of malonaldehyde,proline,hydrogen peroxide,and total flavonoids as well as activities of catalase(CAT),peroxisome(POD),superoxide dismutase(SOD),ascorbate peroxidase(APX),and glutathione reductase(GR)of the plants were monitored by biochemical methods,those of plant hormones,including abscisic acid,jasmonic acid,auxin,and salicylic acid,measured by ELISA and HPLC,and expressions of the genes associated with antioxidant enzymes and salicylic acid signal transduction and synthesis analyzed by RT-qPCR.[Result]Six days after infestation by BPHs,all TN1 seedlings died but BP4 had a mortality rate of merely 11.11%with a rated Level 3 resistance to the pest.In 4 d after the exposure,both germplasms had significantly increased contents of malonaldehyde and proline without any changes on the total flavonoids;TN1,but not BP4,rose significantly on hydrogen peroxide;TN1 increased significantly on the activities of CAT,POD,and SOD,but BP4 decreased significantly;neither germplasms changed on APX activity;TN1,but not BP4,declined significantly on GR activity;and only BP4 increased significantly on the content of salicylic acid.In 2 d post-introduction,the expressions of most antioxidant enzyme-associated genes in BP4 were downregulated but upregulated in TN1.Meanwhile,the expressions of the genes related to the salicylic acid signal transduction and synthesis in BP4 were upregulated.[Conclusion]BPH infestation induced significant differentiations between the resistant BP4 and the susceptible TN1 rice germplasms in response to the stress with respect to the contents of hydrogen peroxide and salicylic acid as well as the antioxidant enzyme activity of the plants.关键词
水稻/褐飞虱/应激反应/抗氧化酶/水杨酸Key words
rice/brown planthopper/stress response/antioxidant enzyme/salicylic acid分类
农业科技引用本文复制引用
连玲,董萌,吴春珠,张居念,施龙清,解振兴,陈玉玲,吴扬,姜照伟..抗感虫水稻受褐飞虱取食诱导的应激反应差异性分析[J].福建农业学报,2025,40(5):449-458,10.基金项目
国家自然科学基金项目(32301815) （32301815）
福建省自然科学基金项目(2022J01449) （2022J01449）
福建省农业科学院农业科技专项(GJYS202405) （GJYS202405）
