福建农业学报2025，Vol.40Issue(5)：459-466,8.DOI:10.19303/j.issn.1008-0384.2025.05.004
关岭牛SIRT3基因多态性与体尺、体重性状的关联分析
Correlation between SIRT3 Polymorphisms and Growth Indicators of Guanling Cattle
摘要
Abstract
[Objective]Correlation between the polymorphisms of sirtuin 3-associated gene(SIRT3)in metabolically active tissues and the growth indicators of Guanling cattle was analyzed.[Methods]Tissue samples from the heart,liver,spleen,lung,kidney,leg muscle,longissimus dorsi,and shoulder muscle were collected from three 18-month-old Guanling bulls.Expressions of SIRT3 in the organs and muscles were analyzed by qRT-PCR.A whole-genome resequencing was conducted on the population of 187 Guanling cattle to identify the single nucleotide polymorphism(SNP)loci in SIRT3.Association of the genetic variants with the cattle growth traits was evaluated using a mixed linear model.[Results]The SIRT3 gene was ubiquitously expressed in the hindlimb muscle,longissimus dorsi muscle,spleen,lung,kidney,heart,shoulder hump,and liver of Guanling cattle.The longissimus dorsi muscle and shoulder hump had the highest level of expression,followed by the hindlimb muscle and relatively low in the heart,kidney,spleen,and lung.Two SNPs,i.e.,g.8779 C＞T in Exon 6 and g.23000 C＞T in Exon 10,were identified.Significant correlations between the SNPs and the growth of Guanling cattle were observed at the g.8779 C＞T locus in the manner that the cattle of CC genotype exhibited significantly greater withers height than the TT genotype(P＜0.05)as well as significantly larger cannon bone circumference than the CT genotype(P＜0.05).And at the g.23000 C＞T locus,the TT genotype cattle had significantly greater withers height and ischial end width than the CC genotype(P＜0.05)and also wider chest than the CT counterparts(P＜0.05).[Conclusion]The identified SNPs in SIRT3 significantly correlated with certain growth traits of Guanling cattle indicating a potential application of them as genetic markers for breeding purposes.关键词
关岭牛/SIRT3基因/基因多态性/组织表达Key words
Guanling cattle/SIRT3 gene/gene polymorphism/expression in tissues分类
农业科技引用本文复制引用
吕艳丽,蔡福陶,敖叶,周迪,杨蓉,李波,韩改苗,何仕荣,王玲,王珍梅,吴雨..关岭牛SIRT3基因多态性与体尺、体重性状的关联分析[J].福建农业学报,2025,40(5):459-466,8.基金项目
2024年贵州省农业农村厅畜牧发展处项目(贵州地方黄牛参考基因组的建立及相关优异基因鉴定) （贵州地方黄牛参考基因组的建立及相关优异基因鉴定）
贵州省高层次创新型人才项目(黔科合平台人才-GCC[2023]028) （黔科合平台人才-GCC[2023]028）
