福建农业学报2025，Vol.40Issue(5)：487-499,13.DOI:10.19303/j.issn.1008-0384.2025.05.007
枇杷WRKY基因家族鉴定及其表达分析
Identification and Expressions of WRKY Family in Eriobotrya japonica
摘要
Abstract
[Objective]One of the largest transcription factor families in plants that plays an important role in growth,development,and stress responses,WRKYsof Eriobotrya japonica were identified,their expressions examined,and peel pigmentation-related functions determined.[Method]Based on the genome data,EjWRKYs were identified using bioinformatic methods with their characteristics analyzed.Expressions of the genes in the peel pigmentation of Jiefangzhong and Shanpai 3 loquats were determined based on the transcriptome data.qRT-PCR was employed to verify the expressions of some differentially expressed EjWRKYs.[Result]One hundred WRKYs harboring typical domains were identified with the codes of EjWRKY1to EjWRKY100.The length of EjWRKY ranged 150-732 amino acids with molecular weights ranging 17 189.36-79 765.13 Da and isoelectric points ranging 4.92-10.25.Most of the identified WRKYs located in the nucleus with 97 of them unevenly distributed in 17 chromosomes.The three domains and zinc-finger motifs-based classes of the proteins were mostly expressed in the peel of Jiefangzhong and Shanpai 3 loquats.The qRT-PCR and RNA-seq analyses showed agreeable results on EjWRKYs.[Conclusion]One hundred EjWRKYs were identified to be associated with the pigmentation of loquat peel.关键词
枇杷/WRKY/基因家族/表达分析/果皮着色Key words
Eriobotrya japonica/WRKY/gene family/expression analysis/peel pigmentation分类
农业科技引用本文复制引用
张雅玲,邓朝军,苏文炳,陈秀萍,蒋际谋,郑少泉..枇杷WRKY基因家族鉴定及其表达分析[J].福建农业学报,2025,40(5):487-499,13.基金项目
福建省科技计划公益类专项(2022R1028008) （2022R1028008）
福建省自然科学基金项目(2022J01474) （2022J01474）
