福建农业学报2025，Vol.40Issue(5)：518-526,9.DOI:10.19303/j.issn.1008-0384.2025.05.010
不同荔枝资源叶片结构及炭疽菌侵染后生理响应差异
Leaf Structure and Physiological Responses of Litchi Plants to Anthracnose
摘要
Abstract
[Objective]Leaf structure and physiology of resistant and susceptible Litchi chinensis Sonn.in response to anthracnose were compared for improved disease prevention and resistant variety breeding.[Methods]Paraffin sectioned mature leaves of the resistant Haiken 5(HK5)and the susceptible Nongmei 5(NM5H)litchi were examined under a microscope.Wax content in the leaves was determined by chloroform extraction.Collected leaves were inoculated with Colletotrichum gloeosporioides Penz.on mycelial agar disks as treatment and on a PDA medium as control for microscopic observations with he leaves stained with aniline blue at 0,6,and 24 h post inoculation(hpi).Activities of malondialdehyde(MDA),peroxidase(POD),superoxide dismutase(SOD),phenylalanine ammonia lyase(PAL),chitinase,polyphenol oxidase(PPO),and laccase(Lac)of the leaves were analyzed as well.[Results]The structures of the HK5 and NM5H leaves were generally similar.The leaves and spongy tissue of NM5H were significantly thicker than those of HK5,and the HK5 leaves had a significantly higher wax content than NM5H.The appearances of disease spots on the leaves of HK5 and NM5H became significantly different at 24 hpi.The PAL activity rose significantly at 6 hpi,and POD at 24 hpi in HK5;PPO and Lac at 6 hpi,while POD at 24 hpi,in NM5H increased significantly;and Lac significantly increased at 6 hpi and chitinase at 24 hpi in both varieties.[Conclusion]The wax content and thicknesses of leaf and spongy tissue seemed to reflect the anthracnose resistance of the litchi plants.The enzymes,such as PAL,POD,chitinase,PPO,and Lac,in the leaves appeared to play an important role in the mechanism.关键词
荔枝/炭疽病/叶片结构/生理变化/酶活性Key words
Litchi chinensis Sonn./anthracnose/leaf structure/physiological change/enzyme activity分类
农业科技引用本文复制引用
吴佶,曹学仁,张蕾,林祺英,李焕苓,李芳,王家保..不同荔枝资源叶片结构及炭疽菌侵染后生理响应差异[J].福建农业学报,2025,40(5):518-526,9.基金项目
中央级公益性科研院所基本科研业务费专项(1630032022007) （1630032022007）
海南省重点研发计划项目(ZDYF2021XDNY159) （ZDYF2021XDNY159）
国家荔枝龙眼产业体系建设专项(CARS-32-01) （CARS-32-01）
海南省荔枝产业技术体系建设专项(HNARS-08) （HNARS-08）
