福建中医药2025，Vol.56Issue(5)：31-37,7.DOI:10.13260/j.cnki.jfjtcm.2025.05007
基于NF-κB信号通路探讨莲心碱对高脂饮食诱导代谢性高血压小鼠血管炎症的影响
Effect of Liensinine on Vascular Inflammation in High-Fat Diet-Induced Metabolic Hypertensive Mice Based on NF-κB Signaling Pathway
摘要
Abstract
Objective:To investigate the effect of liensinine on vascular inflammation in high-fat diet-induced hypertensive mice based on NF-κB signaling pathway.Methods:Thirty male C57BL/6 mice were divided into control group of 6 mice fed with regular diet,and the remaining 24 mice were used as modeling group fed with high-fat diet,using a random number table method.After 6 weeks of feeding,when the body weight of the modeling group exceeded that of the control group by 20%and systolic blood pressure(SBP)was greater than 120 mm Hg,the metabolic hypertension model was considered successfully established.Twelve suc-cessfully modeled mice were randomly divided into model group and intervention group,with 6 mice in each group.On the second day after successful modeling,the intervention group was intraperitoneally injected with 5 mg/(kg·d)of liensinine,while the control and model groups were intraperitoneally injected with 0.15 mL/(kg·d)of physiological saline,once every 3 days,for 12 consecutive weeks of intervention.During the intervention period,the model and intervention groups were continuously fed high-fat diet.During the experiment,the body weight of mice was measured and recorded weekly,and the systolic blood pressure(SBP),diastolic blood pressure(DBP),and mean arterial pressure(MAP)of mice were measured every 2 weeks using a non-invasive tail blood pressure monitor.At the end of the intervention,retinal venous blood and abdominal aortic tissue were collected.The levels of serum total cholesterol(TC),triglycerides(TG),and low-density lipoprotein cholesterol(LDL-C)were detected.HE staining was used to ob-serve the pathological morphology of the abdominal aorta tissue,and the thickness of the abdominal aorta vascular wall was mea-sured.Immunohistochemistry was used to detect the protein expression levels of interleukin(IL)-1β,tumor necrosis factor-α(TNF-α),IL-6,nuclear factor-κB p65(p65),phosphorylated p65(p-p65),inhibitor of nuclear factor-κB(IκB),and phosphorylated IκB(p-IκB)in abdominal aortic tissue.Results:The HE staining results showed that the control group had intact abdominal aortic tissue structure and no pathological changes were observed;the elastic fibers in the abdominal aortic vessels of the model group were disor-dered arrangement and broken;the elastic fibers in the abdominal aortic vessels of the intervention group were well-organized and in-tact.Compared with the control group,the body weight and SBP of the model group increased significantly from the second week(P<0.05),and DBP and MAP increased significantly from the fourth week(P<0.05);after intervention,the thickness of the abdomi-nal aorta vascular wall increased significantly(P<0.05),and the positive expression rates of IL-1β,TNF-α,IL-6 and the ratios of p-p65/p65 and p-IκB/IκB in the abdominal aortic tissue increased significantly(P<0.05).Compared with the model group,the interven-tion group showed a significant decrease in SBP and MAP from the eighth week(P<0.05),a significant decrease in body weight from the ninth week(P<0.05),and a significant decrease in DBP from the tenth week(P<0.05);after intervention,the thickness of the abdominal aorta vascular wall reduced significantly(P<0.05),and the positive expression rates of IL-1β,TNF-α,IL-6 and the ra-tios of p-p65/p65 and p-IκB/IκB in the abdominal aortic tissue reduced significantly(P<0.05).Conclusion:Liensinine can reduce blood pressure and improve vascular inflammation in metabolic hypertensive mice induced by high-fat diet,and its mechanism may be through the NF-κB signaling pathway.关键词
代谢性高血压/莲心碱/血管炎症/NF-κB信号通路/高脂饮食/小鼠Key words
metabolic hypertension/liensinine/vascular inflammation/NF-κB signaling pathway/high-fat diet/mice引用本文复制引用
姚琳,胡俊灵,郑梦婷,任晨曦,赵小雪,陈达鑫,林珊..基于NF-κB信号通路探讨莲心碱对高脂饮食诱导代谢性高血压小鼠血管炎症的影响[J].福建中医药,2025,56(5):31-37,7.基金项目
福建省自然科学基金项目(2022J01369) （2022J01369）
福建省高校产学合作项目(2024Y4009) （2024Y4009）
福建中医药大学校管课题(X2024036) （X2024036）
