法医学杂志2025，Vol.41Issue(2)：152-159,8.DOI:10.12116/j.issn.1004-5619.2024.440411
铁死亡在α-鹅膏毒肽诱导肝细胞损伤中的作用
The Role of Ferroptosis in Hepatocyte Injury Induced by α-Amanitin
摘要
Abstract
Objective To explore whether ferroptosis is involved in α-amanitin-induced hepatocyte in-jury by detecting iron deposition in mice liver tissues,oxidative stress indicators in hepatocytes and L-02 cells,and expressions of ferroptosis-related proteins after α-amanitin exposure.Methods The poi-soning models of α-amanitin C57BL/6J mice and L-02 cell were established.The Lillie ferrous iron staining and Prussian blue staining were used to detect iron deposition;the kits were applied to detect the levels of superoxide dismutase(SOD),catalase(CAT),malondialdehyde(MDA),and glutathione(GSH).Western blotting was performed to analyze expressions of p53,solute carrier family 7 member 11(SLC7A11),and glutathione peroxidase 4(GPX4).Results Compared with the control group,after α-amanitin exposure,positive cell rates of Fe2+and Fe3+in mice liver tissues increased significantly.In the liver tissues of medium(0.35 mg/kg)and high(0.45 mg/kg)dose groups and L-02 cells treated with 1 μmol/L α-amanitin,the level of GSH decreased,the level of MDA increased,and the activities of SOD and CAT decreased significantly.In addition,α-amanitin upregulated the expression of p53 in a concentration-and time-dependent manner and inhibited the expressions of SLC7A11 and GPX4.Con-clusion Ferroptosis plays an important role in α-amanitin-induced hepatocyte injury.Abnormalities of ferroptosis-related indicators can provide references for the forensic identification of α-amanitin poisoning.关键词
法医病理学/法医毒理学/α-鹅膏毒肽/铁死亡/氧化应激/肝损伤/L-02细胞/小鼠Key words
forensic pathology/forensic toxicology/α-amanitin/ferroptosis/oxidative stress/hepatic in-jury/L-02 cell/mice分类
医药卫生引用本文复制引用
王浩伟,张晓星,杨根梦,王尚文,曾晓锋..铁死亡在α-鹅膏毒肽诱导肝细胞损伤中的作用[J].法医学杂志,2025,41(2):152-159,8.基金项目
云南省科技厅应用基础研究青年项目(202301AU070216) （202301AU070216）
云南省科技厅-昆明医科大学联合专项(202301AY070001-263) （202301AY070001-263）
上海市法医学重点实验室暨司法部司法鉴定重点实验室开放课题资助项目(KF202301) （KF202301）
昆明医科大学博士研究生教育创新基金资助项目(2025B021) （2025B021）
