口腔疾病防治2025，Vol.33Issue(8)：619-630,12.DOI:10.12016/j.issn.2096-1456.202550163
过表达SAP缓解小鼠牙周炎的机制研究
The mechanism of SAP overexpression in alleviating periodontitis in mice
摘要
Abstract
Objective To investigate the mechanism by which serum amyloid P component(SAP)alleviates peri-odontitis in mice,providing an experimental basis to establish SAP as a novel therapeutic agent for periodontitis.Meth-ods Ethical approval was obtained from the Institutional Animal Ethics Committee.Periodontitis models were estab-lished in wild-type(WT)mice and SAP-transgenic(SAP-Tg)mice,divided into four groups:WT control(WT group),WT periodontitis(WT+P group),SAP-Tg control(Tg group),and SAP-Tg periodontitis(Tg+P group).On day 7,the mice were euthanized,and periodontal tissues,teeth,and alveolar bone were collected.SAP protein expression was detected by enzyme-linked immunosorbent assay(ELISA).Micro-CT and HE staining were used to measure alveolar bone resorp-tion(distance from the cementoenamel junction to the alveolar bone crest).Tartrate-resistant acid phosphatase(TRAP)staining was performed to assess osteoclast number,and immunohistochemistry(IHC)was employed to evaluate macro-phage infiltration.The expression levels of inflammatory cytokines including interleukin-1β(IL-1β),interleukin-6(IL-6),and tumor necrosis factor-α(TNF-α)were measured by qRT-PCR.Oral microorganism composition was analyzed using 16S ribosomal RNA(16S rRNA)gene sequencing.Additionally,macrophages from WT and SAP-Tg mice were isolated to establish an in vitro inflammation model,divided into WT+LPS and Tg+LPS groups.The expression of mac-rophage polarization-related genes including inducible nitric oxide synthase(iNOS),CD86,CD163,and CD206)were assessed by qRT-PCR.After the induction of osteoclast differentiation,TRAP staining was performed.Results ELISA results demonstrated that periodontal tissues from Tg+P group mice exhibited higher levels of SAP expression compared to the WT+P group.Micro-CT and HE staining analyses revealed that the Tg+P group showed reduced alveolar bone re-sorption,indicated by a shorter distance between the cementoenamel junction and alveolar bone crest,compared to the WT+P group.Furthermore,TRAP staining results indicated a decrease in osteoclast numbers in the Tg+P group com-pared to the WT+P group.IHC and qRT-PCR results indicated reduced macrophage infiltration and decreased expres-sion of IL-1β,IL-6,and TNF-α in the Tg+P group.Oral microorganism sequencing showed no significant difference in periodontitis-associated pathogenic bacteria between WT+P and Tg+P groups.In vitro experiments demonstrated that compared to the WT+LPS group,the Tg+LPS group exhibited downregulated M1 macrophage markers(iNOS and CD86)and upregulated M2 macrophage markers(CD163 and CD206).TRAP staining confirmed fewer osteoclasts in the Tg+LPS group.Conclusion SAP overexpression effectively alleviates periodontitis severity in mice by inhibiting M1 mac-rophage polarization,reducing pro-inflammatory cytokine expression,and suppressing osteoclast differentiation,thereby attenuating alveolar bone resorption.关键词
血清淀粉样P物质/牙周炎/炎症因子/巨噬细胞极化/16SrRNA基因测序/微生物/破骨细胞/牙槽骨吸收Key words
serum amyloid P component/periodontitis/inflammatory factors/macrophages polarization/16S ribosomal RNA gene sequencing/microorganism/osteoclast/alveolar bone resorption分类
医药卫生引用本文复制引用
黄茵茵,梁栋梁,邹耀堃,韩景如,葛青,刘雪妍,郭亚东,黄新丽,杨岚..过表达SAP缓解小鼠牙周炎的机制研究[J].口腔疾病防治,2025,33(8):619-630,12.基金项目
广东省医学科研基金项目(A2021412) （A2021412）
广东省基础与应用研究基金(2021A1515110988) This study was supported by the grants from Guangdong Provincial Medical Research Foundation Project(No.A2021412) （2021A1515110988）
Guangdong Provincial Basic and Applied Research Foundation(No.2021A1515110988). （No.2021A1515110988）
