广东医学2025，Vol.46Issue(6)：818-825,8.DOI:10.13820/j.cnki.gdyx.20243357
成分鉴定和肠单细胞测序联合分析四君子汤靶向TLR4/NF-κB通路对应激相关肠损伤的影响
Component identification and integrated ileal single-cell sequencing analysis reveal the protective role of Sijunzi Decoction against stress-induced intestinal injury via targeting the TLR4/NF-κB pathway
摘要
Abstract
Objective To investigate the molecular mechanisms by which Sijunzi Decoction(SJZ)ameliorates stress-induced intestinal mucosal injury(SII)in a severe burn mouse model through a combined strategy of mass spec-trometry-based component identification and ileal single-cell RNA sequencing analysis.Methods A mouse model of stress-induced intestinal injury following severe burn was established.Active compounds in SJZ were identified via mass spectrometry,and ileal single-cell transcriptomic analysis was performed.Potential molecular targets of SJZ in SII were screened through integrated bioinformatics analysis,and validated by histopathological examination,immunofluorescence,and qPCR.Results A total of 2 415 chemical constituents were detected in SJZ,including peptides,tri terpenoids,and flavonoids.Specifically,28 components were identified from Panax ginseng,7 from Atractylodes macrocephala,12 from Poria cocos,and 13 from Glycyrrhiza uralensis.Severe burn led to significant reduction in intestinal epithelial cell popula-tions and upregulation of the NF-κB signaling pathway.Integrated analysis identified TLR4 and NF-κB as key targets regulated by SJZ.SJZ treatment reduced epithelial cell loss,increased mucin secretion,and downregulated TLR4 and NF-κB at both mRNA and protein levels compared with the sterile water group.Conclusion SJZ may exert protective effects against stress-induced intestinal injury by suppressing the TLR4/NF-κB signaling pathway and preserving intes-tinal epithelial integrity.关键词
四君子汤/应激后肠黏膜损伤/单细胞测序/TLR4/NF-κB通路Key words
Sijunzi Decoction/stress-induced intestinal mucosal injury/single-cell RNA sequencing/TLR4/NF-κB signaling pathway分类
医药卫生引用本文复制引用
徐浩,肖国中,陈华显,林宏城,贺秋兰..成分鉴定和肠单细胞测序联合分析四君子汤靶向TLR4/NF-κB通路对应激相关肠损伤的影响[J].广东医学,2025,46(6):818-825,8.基金项目
国家自然科学基金资助项目(82004368,82405397,82374442) （82004368,82405397,82374442）
