高师理科学刊2025，Vol.45Issue(7)：68-74,7.DOI:10.3969/j.issn.1007-9831.2025.07.013
鸡CPT1A基因的克隆载体构建及生物信息学分析
Cloning vector construction and bioinformatics analysis of chicken CPT1A gene
摘要
Abstract
Carnitine palmitoyl transferase 1A(CPT1A)is the rate-limiting enzyme of mitochondrial fatty acid β oxidation and plays a key role in energy metabolism.The full-length CDS sequence of chicken CPT1A gene was obtained by RT-PCR,and the cloning vector of the gene was successfully constructed by directional cloning.The differential expression of the gene in 16 tissues and cells such as chicken abdominal fat,muscle stomach fat,Immortalized chicken preadipocyte cell 1 and Intramuscular preadipocytes was detected by RT-QCPR.The protein structure,subcellular localization and phylogenetic relationship were predicted by bioinformatics tools.The results showed that the total length of chicken CPT1A gene ORF was 2 313 bp,encoding 770 amino acids.CPT1A protein has no signal peptide and has two transmembrane domains,and subcellular localization is in cytoplasm and mitochondrial membrane,so it belongs to hydrophilic stable protein.The secondary structure mainly exists in the form of α helix.Phylogenetic tree shows the closest relationship with birds.The study provides a molecular basis for the functional study of CPT1A in chicken lipid metabolism.关键词
肉碱棕榈酰转移酶1A/CPT1A基因/脂肪酸代谢/脂质代谢Key words
carnitine palmitoyl transferase 1A/CPT1A gene/fatty acid metabolism/lipid metabolism分类
生物科学引用本文复制引用
王园园,张越,李淑玲,胡笳,李美琦,刘殿辉,孙婴宁..鸡CPT1A基因的克隆载体构建及生物信息学分析[J].高师理科学刊,2025,45(7):68-74,7.基金项目
黑龙江省省属本科高校优秀青年教师基础研究支持计划资助(YQJH2023103) （YQJH2023103）
黑龙江省自然科学基金(LH2024C124) （LH2024C124）
齐齐哈尔大学大学生创新创业训练计划项目(S202410232025) （S202410232025）
