西部中医药2025，Vol.38Issue(6)：5-12,8.DOI:10.12174/j.issn.2096-9600.2025.06.02
CB1R/BDNF调控异相睡眠剥夺诱发术后疼痛敏化的机制研究
Study on the Regulatory Mechanism of CB1R/BDNF in Pain Sensitization after Paradoxical Sleep Deprivation
摘要
Abstract
Objective:To evaluate whether Cannabinoid type-1 receptor(CB1R)of RVM is involved in the regulation of incision pain sensitization induced by paradoxical sleep deprivation(PSD),and the interaction of BDNF and CB1R in the regulation of pain.Methods:All 96 male SPF-grade SD rats were divided into the blank group(C group),incision group(I group),and PSD group(P group),PSD-incision group(P-I group),PSD-incision+CB1R antagonist group(P-I+SR group),and PSD-incision+CB 1R agonist group(P-I+WIN group),16 rats in each group,sleep deprivation models with plantar incision were established,P-I+SR group,and P-I+WIN group accepted the injection of CB1R inhibitors or CB1R agonists into RVM of the rats on the first day after plantar incision.Immunofluorescence staining,WB,qPCR and other molecular biological methods were used to detect the expressions of BDNF and CB1R protein and mRNA in RVM.Results:Compared with I group,pain sensitization and the duration of postoperative pain were prolonged(P＜0.05).Compared with P-I group,the decrease of CPS and the increase of PWT were found on the first,forth and seventh day after the operation in P-I+WIN group(P＜0.05),while the increase of CPS and the decrease of PWT were seen on the first,forth and seventh day after the operation in P-I+SR group(P＜0.05).Conclusion:Activation of CB1R in RVM reduces incision pain sensitization induced by preoperative PSD,and the interaction between CB1R and BDNF may play a role in promoting downstream modulation.关键词
异相睡眠剥夺/术后疼痛/延髓头端腹内侧部/大麻素1型受体/脑源性神经营养因子Key words
paradoxical sleep deprivation/postoperative pain/rostral ventromedial medulla/cannabinoid type-1 receptor/brain-derived neurotrophic factor分类
医药卫生引用本文复制引用
李文娟,周婷,张杰,薛建军..CB1R/BDNF调控异相睡眠剥夺诱发术后疼痛敏化的机制研究[J].西部中医药,2025,38(6):5-12,8.基金项目
甘肃省自然科学基金(21JR7RA586). （21JR7RA586）
