西部中医药2025，Vol.38Issue(6)：29-33,5.DOI:10.12174/j.issn.2096-9600.2025.06.06
七叶皂苷钠对腰椎间盘突出症大鼠背根神经节VGSCs表达的影响
Influence of Sodium Aescinate Injection on the Expressions of VGSCs in Dorsal Root Ganglion of LDH Rats
摘要
Abstract
Objective:To observe the effects of epidural injection of sodium aescinate on the expressions of voltage-gated sodium channels(VGSCs)and related inflammatory factors in the neuronal cells of dorsal root ganglion(DRG)of the rats with lumbar disc herniation(LDH).Methods:All 32 male SD rats were randomized into the blank group,sham operation group,nucleus pulposus group and sodium aescinate group,eight rats in each group.The intervenstion started after successfully modeling,sodium aescinate group was intervened at a dose of 0.065 mg/kg,other three groups remainded unhandled and they were on a free diet.After the intervention,ELISA was used to detect the expressions of TNF-α and IL-6 in DRG of the rats in different groups,RT-PCR was applied to detect the expressions of Nav1.3,Nav1.7 and Nav1.8 mRNA in DRG of the rats in different groups;and HE was adopted to observe histomorphological changes of DRG of the rats,to compare paw withdrawal threshold(PWT)and thermal pain thresholds of rats in each group on the day before modeling,the first,fifth,tenth and fourteenth day after modeling.Results:Compared with nucleus pulposus group,the improvements of histomorphological structure of DRG were evident in sodium aescinate group,PWT and thermal pain thresholds were elevated(P＜0.05),and the expressions of Nav1.3,Nav1.7 and Nav1.8mRNA,and the levels of TNF-α and IL-6 were reduced in DRG tissue(P＜0.05).Conclusion:Epidural injection of sodium aescinate has certain analgesic effects on LDH model rats,and its mechanism may be related to the inhibition of the levels of Nav1.3,Nav1.7 and Nav1.8,TNF-α and IL-6 by the medicine.关键词
椎间盘突出症,腰/背根神经节/电压门控钠通道/七叶皂苷钠Key words
intervertebral disc herniation,lumbar/dorsal root ganglion/VGSCs/sodium aescinate分类
医药卫生引用本文复制引用
王培鑫,赵军,徐世红,王兴盛,李玙璠,王晓帅,张辰,朱欢,袁剑..七叶皂苷钠对腰椎间盘突出症大鼠背根神经节VGSCs表达的影响[J].西部中医药,2025,38(6):29-33,5.基金项目
甘肃省自然科学基金(20JR10RA360). （20JR10RA360）
