神经损伤与功能重建2025，Vol.20Issue(8)：435-440,6.DOI:10.16780/j.cnki.sjssgncj.20250816
丁苯酞抑制线粒体氧化应激改善卒中后血脑屏障功能
DL-3-n-Butylphthalide Attenuates Mitochondrial Oxidative Stress and Improves Blood-Brain Barrier Function After Stroke
摘要
Abstract
Objective:To investigate the effect and mechanism of Dl-3-n-butylphthalide(NBP)on promoting neurological function recovery in mice with acute ischemic stroke(AIS).Methods:(1)An in vivo middle cerebral artery occlusion(MCAO)mouse model was established,with three groups:Sham,MCAO,and MCAO+NBP.Behavioral assessments,immunofluorescence staining,and transmission electron microscopy were used to evaluate the protective effects of NBP on blood-brain barrier(BBB)integrity and neurological function.(2)An in vitro oxygen-glucose deprivation/reoxygenation(OGD/R)model was established in brain microvascular endothelial cells,followed by NBP treatment.Endothelial function and mitochondrial status were assessed through migration and tube formation assays,permeability tests,CCK-8 assay,propidium iodide staining,and fluorescence probe staining of cell slides.Results:(1)Compared with the MCAO group,NBP treatment significantly increased the expression of endothelial tight junction proteins Claudin-5,ZO-1,and Occludin,reduced leakage of plasma proteins albumin and fibrinogen,decreased levels of the mitochondrial DNA oxidative damage marker 8-OHdG,and improved neurological function(P<0.05).(2)In vitro,NBP treatment enhanced endothelial cell migration and tube formation,reduced permeability,improved cell viability,and decreased DNA damage.In addition,mitochondrial activity,membrane potential,and oxidative stress levels were significantly improved(P<0.05).Conclusion:NBP exerts protective effects in AIS by attenuating mitochondrial oxidative stress,preserving endothelial cell function,restoring BBB integrity,and thereby promoting neurological recovery.关键词
丁苯酞/急性缺血性卒中/血脑屏障/线粒体氧化应激/神经功能恢复Key words
DL-3-n-butylphthalide/acute ischemic stroke/blood-brain barrier/mitochondrial oxidative stress/neurological recovery分类
医药卫生引用本文复制引用
田梦露,许瑶,初云惠,肖君,尚珂,田代实,秦川..丁苯酞抑制线粒体氧化应激改善卒中后血脑屏障功能[J].神经损伤与功能重建,2025,20(8):435-440,6.基金项目
国家自然科学基金项目(血管相关小胶质细胞的脂质感应和清除途径:慢性低灌注脑白质损伤的调控新靶点?No.82271341) （血管相关小胶质细胞的脂质感应和清除途径:慢性低灌注脑白质损伤的调控新靶点?No.82271341）
