神经损伤与功能重建2025，Vol.20Issue(8)：459-464,6.DOI:10.16780/j.cnki.sjssgncj.20241418
通督御疲调神电针调节线粒体功能改善慢性疲劳综合征认知障碍的机制研究
Study on the Mechanism of Tongdu Yupi Tiaoshen Electric Acupuncture Improving Cognitive Impairment in Chronic Fatigue Syndrome by Regulating Mitochondrial Function
摘要
Abstract
Objective:This study aimed to explore the efficacy of Tongdu Yupi Tiaoshen electric acupuncture on cognitive impairment in rats with chronic fatigue syndrome(CFS)and its impact on mitochondrial fission and mitophagy in the hippocampal CA1 region.Methods:A CFS cognitive impairment rat model was established using a chronic fatigue multifactorial compound stress method.The successfully modeled rats were randomly divided into a model group and an electroacupuncture group,with 10 rats in each,while another 10 healthy male SD rats served as the control group.During the treatment period,the control group was fed normally,the model group continued to receive modeling stimulation,and the electroacupuncture group received additional electroacupuncture treatment at the GV24 puncture GV20 and the GV14,with a continuous wave,frequency 10 Hz,15 minutes per session per day,for a consecutive 28 days.The general condition and learning and memory abilities of rats in each group were evaluated using the General Conditions Semi-Quantitative Observation Scale and Morris water maze;transmission electron microscopy was used to observe the ultrastructure of mitochondria in the hippocampal CA1 region of rats in each group;Western Blot and RT-qPCR were used to detect the protein and mRNA expression levels of key molecules Pink1,Parkin,and Drp1 related to mitochondrial autophagy and mitochondrial fission in the hippocampal CA1 region of rats in each group.Results:After treatment,compared with the control group,the general condition score of the model group was increased(P<0.05),the escape latency was prolonged(P<0.05),and the number of platform crossings was reduced(P<0.05);mitochondrial damage in the hippocampal CA1 region was increased,with swelling or shrinking in shape,lightened matrix with vacuolization,disappearance of cristae,incomplete outer membrane,and the appearance of autophagic bodies;the protein and mRNA expression levels of Pink1,Parkin,and Drp1 were decreased(P<0.05).After treatment,compared with the model group,the general condition score of the electroacupuncture group was decreased(P<0.05),the escape latency was shortened(P<0.05),and the number of platform crossings was increased(P<0.05);there were fewer damaged mitochondria and autophagic bodies in the hippocampal CA1 region,with mildly swollen shape,relatively clear inner cristae,cristae fracture but no disappearance of cristae protrusions,roughly intact outer membrane,and no significant change in matrix;the protein and mRNA expression levels of Pink1,Parkin,and Drp1 were upregulated(P<0.05).Conclusion:Tongdu Yupi Tiaoshen electric acupuncture has anti-fatigue and cognitive-improving effects,which may be related to the regulation of mitochondrial autophagy and mitochondrial fission function in CFS cognitive impairment rats.关键词
慢性疲劳综合征/认知障碍/线粒体分裂/线粒体自噬/电针Key words
chronic fatigue syndrome/cognitive impairment/mitochondrial division/mitochondrial autophagy/electroacupuncture分类
医药卫生引用本文复制引用
冯楚文,鲁菁,屈媛媛,孙维伯,刘婷婷,吴永芳,杨添淞..通督御疲调神电针调节线粒体功能改善慢性疲劳综合征认知障碍的机制研究[J].神经损伤与功能重建,2025,20(8):459-464,6.基金项目
黑龙江省自然科学基金(电针调控线粒体动力学促进CFS认知障碍大鼠突触重塑的机制研究,No.LH2024H050) （电针调控线粒体动力学促进CFS认知障碍大鼠突触重塑的机制研究,No.LH2024H050）
国家自然科学基金(电针调控MDM2泛素化PSD-95水平重塑海马神经元突触结构改善CFS认知功能障碍的机制研究,No.82074539 （）
艾灸激活CaMKⅡ磷酸化介导长时程增强调节突触可塑性治疗CFS认知障碍的机制研究,No.82305394) （）
教育部"春晖计划"合作科研项目("通督御疲"调神针法改善CFS认知功能障碍海马突触可塑性的效应机制研究,No.HZKY20220308-202201357) （"通督御疲"调神针法改善CFS认知功能障碍海马突触可塑性的效应机制研究,No.HZKY20220308-202201357）
黑龙江省中医药科研课题(电针调节CFS认知功能障碍大鼠海马突触可塑性的作用机制研究,No.ZHY2022-136) （电针调节CFS认知功能障碍大鼠海马突触可塑性的作用机制研究,No.ZHY2022-136）
黑龙江省中医药学会青年人才托举工程项目("通督御疲调神针法"改善CFS认知功能障碍海马突触可塑性的效应机制研究,No.2022-QNRC1-05) （"通督御疲调神针法"改善CFS认知功能障碍海马突触可塑性的效应机制研究,No.2022-QNRC1-05）
黑龙江省青年岐黄学者培养项目(黑中医药教函[2023]36号). （黑中医药教函[2023]36号）
