高原农业2025，Vol.9Issue(4)：421-431,11.DOI:10.19707/j.cnki.jpa.2025.04.001
水曲柳枝枯病病病原菌分离鉴定、生物学特性及发病前后真菌多样性分析
Isolation,Identification,Biological Characteristics,and Analysis of Fungal Diversity before and after the Onset of Fraxinus mandshurica Shoot Blight by High-throughput Sequencing
摘要
Abstract
Through tissue isolation and purification of the collected diseased branches,representative pathogenic strains were obtained.Pathogenicity was verified by Koch's rule,and the types of pathogens were identified by combining morphology and molecular biology;Through different culture media,different temperature,different pH and different lighting conditions,a preliminary study was conducted on the biological characteristics of pathogen.The high-throughput sequencing was used to investigate the differences in fungal community structures between diseased skin and healthy skin before and after the occurrence of F.mandshurica shoot blight.This study aimed to provide scientific theoretical guidance for the field diagnosis and prevention of F.mandshurica shoot blight,with strong practical significance.Six strains were obtained after isolation and purification,and the pathogenicity of strain FL1 was consistent with field symptoms;by combining morphology and molecular biology,the pathogen causing F.mandshurica shoot blight was identified as D.cotoneastri;under artificial cultivation conditions,the optimal growth environment for the strain was PDA medium,25℃,pH=6,and 12L/12D.The fungal ASV changed greatly before and after susceptibility to disease.The two samples shared 86 fungal species,accounting for 16.96%,with two substantially identical sample colonies structures,but large differences in relative abundance.At the phylum level,Ascomycota was the dominant fungus.At the genus level,Didymella was the predominant fungus in healthy skin,and Diaporthe was the predominant fungus in diseased skin,followed by Didymella.This study confirms that D.cotoneastri causes F.mandshurica shoot blight,and there is a significant change in the number of fungal species before and after infection.关键词
水曲柳枝枯病/病原菌鉴定/生物学特性/真菌多样性Key words
F.mandshurica shoot blight/Pathogen identification/Biological characteristics/Fungal diversity分类
农业科技引用本文复制引用
董爱荣,于文旭,王思涵,孙宁..水曲柳枝枯病病病原菌分离鉴定、生物学特性及发病前后真菌多样性分析[J].高原农业,2025,9(4):421-431,11.基金项目
国家自然科学基金项目(31670494). （31670494）
