高原农业2025，Vol.9Issue(4)：451-461,11.DOI:10.19707/j.cnki.jpa.2025.04.005
金黄色葡萄球菌野生株与耐药株的竞争
The Competition between Wild-type Staphylococcus Aureus and Antibiotic-Resistant Strains
摘要
Abstract
This study aimed to explore the competitive dynamics between wild-type and drug-resistant strains of Staphylococcus aureus under antibiotic-free conditions.The wild-type strain ATCC6538 and the resistant strain P01 were co-cultured in liquid medium for 32 hours,and to evaluate the competitive adaptability of the two strains,we monitored the dynamic changes in their viable cell densities in the medium.During separate cultures,both ATCC6538 and P01 reached viable cell densities of 108 to 109 CFU·mL-1.However,in the mixed culture,the viable cell density of strain ATCC6538 reached 108 CFU·mL-1,whereas strain P01 reached 106～107 CFU·mL-1,indicating that in the absence of antibiotic selection pressure,the wild-type strain demonstrated superior competitive fitness compared to the resistant strain.During the early stage of co-culture,both strains exhibited similar changes in cell density,but approximately 12 h into the mixed culture,strain ATCC6538 continued to increase in density,while strain P01 began to decrease,ultimately leading to strain ATCC6538 gaining a competitive advantage,likely due to differences in metabolic efficiency and resource utilization.This suggests that the wild-type strain ATCC6538 exhibited greater competitive capability compared to the resistant strain P01 in the later stages of mixed culture.The development of antibiotic resistance has been shown to impose a metabolic burden on the cells,which can negatively impact cellular growth rates and functional traits,thereby reducing competitive fitness under nutrient-limited conditions.Moreover,changes during the growth process indicate that resource availability is also a crucial factor affecting coexistence and competition between the two strains.These findings provide a reference for further analysis of microbial competitive interactions.关键词
金黄色葡萄球菌/混合培养/竞争/耐药性成本/活菌密度Key words
Staphylococcus aureus/Mixed cultivation/Competition/Cost of drug resistance/Viable bacterial density分类
生物科学引用本文复制引用
张首超,董营,吉毛加,闫刚,张新军..金黄色葡萄球菌野生株与耐药株的竞争[J].高原农业,2025,9(4):451-461,11.基金项目
国家自然科学基金资助项目(31960013) （31960013）
西藏自治区自然科学基金重点项目(XZ202301ZR0047G) （XZ202301ZR0047G）
西藏自治区科技计划项目(XZ202301YD0028C) （XZ202301YD0028C）
西藏高原森林生态教育部重点实验室开放课题项目(XZA-JYBSYS-2024-09,XZA-JYBSYS-2023-15) （XZA-JYBSYS-2024-09,XZA-JYBSYS-2023-15）
西藏农牧学院研究生教育创新计划项目(YJS2024-43). （YJS2024-43）
