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羊肚菌液体菌种培养条件研究

杨田婷 文思博 韦春某

耕作与栽培2025,Vol.45Issue(6):24-27,32,5.
耕作与栽培2025,Vol.45Issue(6):24-27,32,5.

羊肚菌液体菌种培养条件研究

Optimization of Culture Conditions for Liquid Spawn of Morchella spp

杨田婷 1文思博 1韦春某1

作者信息

  • 1. 铜仁职业技术大学,贵州 铜仁 554300
  • 折叠

摘要

Abstract

This paper took the Morchella eximia strain as the material and studied the effects of different culture conditions on its liquid strain by measuring the mycelial biomass and the number of mycelial pellets under various conditions,including the type of culture medium,pH value,temperature,rotation speed of the culture box,inoculation amount,carbon source,and nitrogen source.The results indicated that the suitable medium selected was BM medium,with a pH of 6,a culture tempera-ture of 25℃,a shaker speed of 240 r/min,an inoculation amount of 9 pieces,a carbon source of sucrose,and a nitrogen source of beef extract.

关键词

羊肚菌/液体菌种/培养基

Key words

Morchella spp/liquid strain/culture medium

分类

农业科技

引用本文复制引用

杨田婷,文思博,韦春某..羊肚菌液体菌种培养条件研究[J].耕作与栽培,2025,45(6):24-27,32,5.

基金项目

羊肚菌优良品种选育与驯化栽培关键技术研究(铜仁科研[2023]64号) (铜仁科研[2023]64号)

贵州省高等学校工程研究中心建设(黔教技[2023]051号) (黔教技[2023]051号)

梵净山野生灰树花种质资源筛选及驯化栽培研究(铜仁科研[2024]20号) (铜仁科研[2024]20号)

贵州省食用菌产业技术体系综合试验站(GZMARS-SYJ-2025-2027-10) (GZMARS-SYJ-2025-2027-10)

武陵山区野生食用菌人工驯化与试验示范(黔农菌重点[2024]02) (黔农菌重点[2024]02)

耕作与栽培

1008-2239

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