中医药导报2025，Vol.31Issue(7)：9-15,7.DOI:10.13862/j.cn43-1446/r.2025.07.002
石见穿多糖通过调控miRNA-145抑制胃癌细胞SGC-7901的增殖、凋亡、侵袭及迁移
In Vitro Study on the Inhibition of Proliferation,Apoptosis,Invasion and Migration of Gastric Cancer Cells SGC-7901 by Salvia Chinensis Benth.Polysaccharides through Regulating miRNA-145
摘要
Abstract
Objective:To investigate the effects and mechanisms of Salvia Chinensis Benth.Polysaccharides(SCBP)on the proliferation,apoptosis,invasion and migration of SGC-7901 cells.Methods:The suitable SCBP concentration was chosen by MTT and cell apoptosis tests.The SGC-7901 cells were divided into normal group(NC group),SCBP group,miRNA group,SCBP+miRNA group and SCBP+miRNA inhibitor group.The cell proliferation,cell apoptosis,cell cycle,invasion cell number and wound healing rate were evaluated by MTT,flow cytometry,transwell and wound healing assays.Western blotting was used to detect the protein expression of phosphatidylinositide 3-kinases(PI3K),protein kinase B(Akt),cyclin-dependent kinase inhibitor 1A(P21),C-MYC protein(c-Myc),matrix metallopeptidase 2(MMP-2)and matrix metallopeptidase 9(MMP-9).Results:The suitable SCBP concentration was 50 μg/mL.The SCBP group,miRNA group,and SCBP+miRNA group showed lower cell proliferation rate,invasion cell number and wound healing rate than NC group(P＜0.05),while higher cell apoptosis and G1 phase rate than NC group(P＜0.05).SCBP+miRNA group showed lower cell proliferation rate and wound healing rate than SCBP group(P＜0.05),while higher cell apoptosis and G1 phase rate than SCBP group(P＜0.05).SCBP+miRNA inhibitor group showed higher cell proliferation rate and wound healing rate than SCBP group(P＜0.01),while lower cell apoptosis and G1 phase rate than SCBP group(P＜0.01).SCBP group,miRNA group,and SCBP+miRNA group showed lower relative expression level of PI3K,Akt,c-Myc,MMP-2 and MMP-9 proteins than NC group(P＜0.05),while higher relative expression level of P21 protein than NC group(P＜0.05).SCBP+miRNA group showed lower relative expression level of PI3K,Akt,c-Myc,MMP-2 and MMP-9 proteins than SCBP group,while higher relative expression level of P21 protein than SCBP group,with statistically significant differences(P＜0.05).SCBP+miRNA inhibitor group showed higher relative expression level of PI3K,Akt,c-Myc,MMP-2 and MMP-9 proteins than SCBP group,while lower relative expression level of P21 protein than SCBP group,with statistically significant differences(P＜0.01).Conclusion:SCBP can inhibit the proliferation,apoptosis,invasion and migration of SGC-7901 by inhibition of PI3K/Akt/c-Myc/MMP-2/MMP-9 signaling pathway activation.关键词
胃癌/石见穿多糖/miRNA-145/细胞凋亡/细胞增殖/细胞侵袭/细胞迁移Key words
gastric cancer/Salvia Chinensis Benth.Polysaccharides/miRNA-145/cell apoptosis/cell proliferation/cell invasion/cell migration分类
医药卫生引用本文复制引用
高峰,王佳,张莉,杨学文..石见穿多糖通过调控miRNA-145抑制胃癌细胞SGC-7901的增殖、凋亡、侵袭及迁移[J].中医药导报,2025,31(7):9-15,7.基金项目
国家中医临床研究基地开放课题(JD201511) （JD201511）
