中医药导报2025，Vol.31Issue(7)：32-39,8.DOI:10.13862/j.cn43-1446/r.2025.07.005
茸脂胶囊治疗激素性股骨头坏死的网络药理学分析及动物实验
Network Pharmacology Analysis and Animal Experiments of Rongzhi Capsules(茸脂胶囊)in the Treatment of Steroid-Induced Osteonecrosis of the Femoral Head
摘要
Abstract
Objective:To explore the mechanism of action of Rongzhi capsules in treating steroid-induced osteonecrosis of the femoral head based on network pharmacology analysis and animal experiments.Method:The potential chemical composition and target prediction of Rongzhi capsules were obtained through the traditional Chinese medicine pharmacology database and analysis platform(TCMSP)database.GeneCards and OMIM databases were used to screen genes related to steroid-induced osteonecrosis of the femoral head,and R software was applied to achieve mapping and matching of drug targets and disease targets.On this basis,a visualization network of drugs,ingredients,targets,and diseases was constructed using Cytoscape software,and a protein interaction network was constructed using the String database online platform.In addition,30 rats were randomly divided into modeling group(n=20)and control group(n=10).A rat model of steroid-induced osteonecrosis of the femoral head was constructed in modeling group,and the successful model rats were randomly divided into model group and Chinese medicine group,10 rats in each group.The Chinese medicine group was administered with Rongzhi capsules by gavage,while the control group and model group rats were given equal volumes of distilled water by gavage.Hematoxylin eosin(HE)staining method was used to observe empty bone cavity rate in rat femoral head in 8 weeks.At the same time,RT qPCR was used to detect the expression of CXC chemokine receptor 2(CXCR2)mRNA,Caspase-3 mRNA,and Bel-2 related X protein(Bax)mRNA in the femoral head of rats.Western blotting techniques was used to detect the protein expression of CXCR2,Caspase-3 and Bax in the femoral head of rats.Result:Totally 163 effective ingredients of Rongzhi capsules were collected,and 578 potential targets for the active ingredients of Rongzhi Capsules were predicted,with 145 potential targets for the treatment of femoral head necrosis.The top 5 potential core target genes were identified as TP53,CXCR2,CTNNB1,EP300 and EGFR.Gene ontology(GO)analysis shows that its biological processes(BP)were mainly enriched in positive regulation of gene expression,RNA polymerase Ⅱ mediated positive transcription regulation,response to hypoxia,negative regulation of gene expression,and positive regulation of interleukin-8(IL-8)production.Its cellular components(CC)were mainly enriched in extracellular space,extracellular regions,collagen containing extracellular matrix,etc.Its molecular functions(MF)were mainly enriched in enzyme binding,homologous protein binding,cytokine active protein homodimerization activity,etc.The Kyoto encyclopedia of genes and genomes(KEGG)pathway mainly involved phosphatidylinositol 3-kinase(PI3K)/protein kinase B(Akt)signaling pathway,FoxO signaling pathway,toll like receptor signaling pathway,interleukin-17(IL-17)signaling pathway,alcoholic liver disease,etc.The Chinese medicine group and model group showed higher empty bone pit rate of the femoral head than control group(P＜0.05).The Chinese medicine group showed lower empty bone pit rate of the femoral head than model group(P＜0.05).The model group showed higher expression of CXCR2 mRNA,Caspase-3 mRNA,Bax mRNA and CXCR2,Caspase-3,Bax protein than control group(P＜0.05).The Chinese medicine group showed lower expression of CXCR2 mRNA,Caspase-3 mRNA,Bax mRNA and CXCR2,Caspase-3,Bax protein than model group(P＜0.05).Conclusion:The characteristics of the therapeutic effect of Rongzhi capsules on steroid-induced osteonecrosis of the femoral head are multi-component and multi-target.CXCR2 is the core target of Rongzhi capsules in treating steroid-induced osteonecrosis of the femoral head.Rongzhi capsules can inhibit the apoptosis of bone cells in rats with steroid-induced osteonecrosis of the femoral head,thereby achieving the therapeutic effect of treating steroid-induced osteonecrosis of the femoral head.关键词
激素性股骨头坏死/茸脂胶囊/网络药理学/CXC趋化因子受体2/分子机制/大鼠Key words
steroid-induced osteonecrosis of the femoral head/Rongzhi capsules/network pharmacology/CXC chemokine receptor 2/molecular mechanisms/rat分类
医药卫生引用本文复制引用
林天烨,邓攀,杨旭辉,陈伯亮,何晓铭,何敏聪,李子祺,侯凯,何伟,张庆文..茸脂胶囊治疗激素性股骨头坏死的网络药理学分析及动物实验[J].中医药导报,2025,31(7):32-39,8.基金项目
广州市科技计划项目(2024A04J5057) （2024A04J5057）
广州中医药大学第三附属医院科研创新基金课题(Sy2022002) （Sy2022002）
陕西省中医药管理局市级中医医院科研能力提升项目(SZY-NLTL-2024-005) （SZY-NLTL-2024-005）
广东省中医药局项目(20223012) （20223012）
