中医药导报2025，Vol.31Issue(7)：46-50,5.DOI:10.13862/j.cn43-1446/r.2025.07.007
越鞠丸类方治疗心衰并抑郁模型的作用及对Elk1-Ghrelin通路的影响
The Effect of Yuejue Wan(越鞠丸)Derivative Formula on Heart Failure and Depression Model and Its Impact on Elk1 and Ghrelin Pathways
摘要
Abstract
Objective:To investigate the therapeutic effects of Yueju Wan derivative formula on heart failure complicated with depression and it's effect on Elk1-Ghrelin pathway.Methods:A mouse model of heart failure complicated with depression was established using left anterior descending coronary artery ligation(LAD)combined with chronic mild stress(CMS).Totally 60 C57BL/6 mice were randomly divided into six groups,including control group,model group,sham operation CMS group,Yueju Wan derivative formula treatment group,AAV-mElk1 group,and AAV-mElk1+Yueju Wan derivative formula treatment group.The therapeutic effects of Yueju Wan derivative formula and its impact on the Elk1-Ghrelin pathway were evaluated through behavioral tests,echocardiography,serological detection,and molecular biology experiments.Results:Yueju Wan derivative formula significantly improved depressive-like behaviors in model mice,increased serum Ghrelin levels,decreased BNP levels,and improved cardiac function indicators(LVEF,LVFS,LVIDd,LVIDs and E/A).Simultaneously,Yueju Wan derivative formula decreased the Elk1 mRNA and Elk1 protein expression levels in hippocampal and cardiac tissues.The Yueju Wan derivative formula treatment group showed the best therapeutic effect.The expression levels of Elk1 in hippocampal tissue(r=-0.78,P＜0.01)and Elk1 in cardiac tissue(r=-0.82,P＜0.01)were negatively correlated with ghrelin.Conclusion:Yueju Wan derivative formula may improve cardiac function and depressive-like behaviors in mice with heart failure complicated with depression by down-regu-lating Elk1expression,thereby increasing serum Ghrelin level.关键词
心力衰竭/抑郁/越鞠丸类方/转录激活因子/ETS样蛋白1/促生长激素释放多肽/小鼠Key words
heart failure/depression/Yueju Wan derivative formula/transcriptional activator/Elk1/Ghre-lin/mouse分类
医药卫生引用本文复制引用
景云佳,朱妍,高昕,蒋健聪..越鞠丸类方治疗心衰并抑郁模型的作用及对Elk1-Ghrelin通路的影响[J].中医药导报,2025,31(7):46-50,5.基金项目
无锡市卫生健康委员会科研项目(Q202354) （Q202354）
