中医药导报2025，Vol.31Issue(7)：57-62,6.DOI:10.13862/j.cn43-1446/r.2025.07.009
基于UPLC-Q-TOF-MS技术和网络药理学探讨阿尔泰金莲花提取物抗炎药效物质及作用机制
Exploring the Anti-Inflammatory Substances and Mechanism of Action of Trollius Altaicus C.A.Mey Extract Based on UPLC-Q-T0F-MS and Network Pharmacology
摘要
Abstract
Objective:To analyze the chemical components of Trollius altaicus C.A.Mey extract and identify its anti-inflammatory active ingredients,targets,and pathways.Methods:Chemical profiling was performed using ultra performance liquid chromatography-quadrupole time-of-flight tandem mass spectrometry(UPLC-Q-TOF-MS).Network pharmacology and molecular docking were combined to predict and validate the active ingredients,targets,and pathways of anti-inflammatory effects.Results:A total of 51 compounds were detected in the extract.The key active ingredients that exert anti-inflammatory effects include orientin,hyperoside and fraxin.The key core targets include epidermal growth factor receptor(EGFR),sarcoma receptor coactivator(SRC),estrogen receptor 1(ESR1),mitogen activated protein kinase 14(MAPK14)and heat shock protein family A member 8(HSPA8).Enrichment analysis revealed 128 signaling pathways.Molecular docking verification showed that the key active ingredients had strong binding affinity with the core target.Conclusion:Trollius altaicus C.A.Mey contains flavonoids,alkaloids,phenolic acids,and other components,and their anti-inflammatory effects are achieved through the synergistic action of multiple components,targets,and pathways.关键词
阿尔泰金莲花/抗炎/活性成分/超高效液相色谱-四级杆串联飞行时间质谱/网络药理学/分子对接Key words
Trollius altaicus C.A.Mey/anti-inflammatory/active substances/ultra performance liquid chromatography-quadrupole time-of-flight tandem mass spectrometry/network pharmacology/molecular dockin分类
医药卫生引用本文复制引用
闫莉,王林洋..基于UPLC-Q-TOF-MS技术和网络药理学探讨阿尔泰金莲花提取物抗炎药效物质及作用机制[J].中医药导报,2025,31(7):57-62,6.基金项目
新疆维吾尔自治区自然科学基金面上项目(2024D01C145) （2024D01C145）
