井冈山大学学报（自然科学版）2025，Vol.46Issue(4)：48-55,8.DOI:10.3969/j.issn.1674-8085.2025.04.006
低温弱光和水杨基氧肟酸处理对番茄幼苗叶绿素荧光成像特性的影响
EFFECTS OF LOW TEMPERATURE WITH LOW LIGHT AND SHAM TREATMENT ON CHLOROPHYLL FLUORESCENCE IMAGING CHARACTERISTICS OF TOMATO SEEDLINGS
摘要
Abstract
The aim of this study was to explore the role of the mitochondrial alternative oxidase(AOX)pathway in alleviating photoinhibition in tomato seedlings subjected to low temperature with low light.In this study,Solanum lycopersicum L.cv Zhongshu No.4 was used as the research material,and the effects of salicylhydroxamic acid(SHAM,1.0 mM)on the chlorophyll fluorescence imaging characteristics of tomato seedlings under low temperature with low light(LT,8 ℃ 200 μmol·m-2·s-1)for 4 d were analyzed.The results showed that LT treatment induced the photoinhibition in tomato seedlings,and its impact on older leaves was greater than that on newly born leaves.LT treatment significantly decreased the relative area of the fluorescence active regions(RAF)and the fluorescence values of whole plant(VW)of Y(II)and qP,but increased the fluorescence values of the fluorescence activity regions(VA)of qP,and also significantly promoted an increase in the VW and VA of Y(NPQ)in tomato seedlings.SHAM treatment did not affect the chlorophyll fluorescence imaging characteristics of tomato seedlings under normal temperature.However,SHAM treatment exacerbated the decrease of Fv/Fm and increased the distribution proportion of the severe photoinhibition region in tomato seedlings under low temperature with low light.SHAM treatment also aggravated the decrease in the RAF and VW of Y(II)and qP,and the increase in the VA of qP in tomato seedlings under low temperature with low light,while the VW and VA decreased slightly.These results implied that low temperature with low light induced the closure of some active PSII reaction centers,and inhibited the PSII photochemistry.AOX pathway played a critical role in alleviating photoinhibition,optimizing the opening degree of the PSII reaction centers and the PSII photochemistry,as well as stabilizing the thermal dissipation pathway in tomato seedlings under low temperature with low light conditions.关键词
番茄/低温弱光/PSII功能/光抑制/叶绿素荧光成像Key words
Solanum lycopersicum L./low temperature with low light/PSII function/photoinhibition/chlorophyll fluorescence imaging分类
农业科技引用本文复制引用
胡文海,曾文悦,王佳慧,罗嘉欣..低温弱光和水杨基氧肟酸处理对番茄幼苗叶绿素荧光成像特性的影响[J].井冈山大学学报（自然科学版）,2025,46(4):48-55,8.基金项目
国家自然科学基金项目(32460752) （32460752）
井冈山大学校级大学生创新创业训练计划项目(JDX2024177) （JDX2024177）
